Impact Of The Gut Microbiota On Host Cells Energy Metabolism in Health And In Inflammatory Bowel Disease (ENERGISED-Clinic)

Conditions

Healthy volunteers : Healthy adults with no history of gastrointestinal disease Patients with IBD in remission

Brief summary

Energy metabolism of major immune cell types for peripheral blood will be assessed on a blood sample. The energy metabolism will be assessed by single-cell energetic metabolism by profiling translation inhibition

Detailed description

Impact of the gut microbiota and its alterations in inflammatory bowel disease on the fate of microbiota-derived carbon sources in host cells: we will use a strategy based on stable isotope probing (SIP). The participants will receive orally food grade inulin from Chicory (2% labelled with 13C) at H0 on day 0 (v1) and day 7 (v2) for healthy adults and day 0 (v1) only for patients with IBD. The incorporation of 13C in metabolites will then be assessed, Identification of the intestinal bacteria responsible for the metabolism of the labeled inulin: we will use SIP-DNA and SIP-RNA sequencing approaches: DNA and RNA will be extracted from each fecal samples obtained

Related papers

No related papers found yet.

Interventions

DRUGAMPHOTERICIN B

DRUGVANCOMYCIN

DRUGGentamicin_APHP

Eligibility

Sex/Gender

All

Age

18 Years to 64 Years

Design outcomes

Primary

Measure	Time frame
Energy metabolism of major immune cell types for peripheral blood will be assessed on a blood sample. The energy metabolism will be assessed by single-cell energetic metabolism by profiling translation inhibition	—

Secondary

Measure	Time frame
Impact of the gut microbiota and its alterations in inflammatory bowel disease on the fate of microbiota-derived carbon sources in host cells: we will use a strategy based on stable isotope probing (SIP). The participants will receive orally food grade inulin from Chicory (2% labelled with 13C) at H0 on day 0 (v1) and day 7 (v2) for healthy adults and day 0 (v1) only for patients with IBD. The incorporation of 13C in metabolites will then be assessed, Identification of the intestinal bacteria responsible for the metabolism of the labeled inulin: we will use SIP-DNA and SIP-RNA sequencing approaches: DNA and RNA will be extracted from each fecal samples obtained	—

Measure

Time frame

Impact of the gut microbiota and its alterations in inflammatory bowel disease on the fate of microbiota-derived carbon sources in host cells: we will use a strategy based on stable isotope probing (SIP). The participants will receive orally food grade inulin from Chicory (2% labelled with 13C) at H0 on day 0 (v1) and day 7 (v2) for healthy adults and day 0 (v1) only for patients with IBD. The incorporation of 13C in metabolites will then be assessed, Identification of the intestinal bacteria responsible for the metabolism of the labeled inulin: we will use SIP-DNA and SIP-RNA sequencing approaches: DNA and RNA will be extracted from each fecal samples obtained

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Countries

France

Outcome results

None listed