Using lymph nOde fine needle aspiration to assess the effeCt of pnEumococcal vAcciNe platform, Age and HIV infection on Germinal cEntre formation (OCEAN-AGE)

Conditions

HIV infection

Brief summary

Immunophenotyping of lymph node FNA samples (% germinal centre B cells and %T follicular helper cells), 4 weeks after vaccination.

Detailed description

Immunophenotyping of PBMC's 6 months after vaccination (% S. pneumoniae PS-specific B cells per serotype, Spn PS-specific B cell phenotype, Spn PS-specific antibody titres, Spn PS-specific antibody fuctionality, CRM-197 specific B cells and T cells., Serum and lymph node supernatant biomarkers at day 7, day 28 and day 72 post vaccination, Immunophenotyping and functional analysis of PBMCs at baseline, Serum biomarkers at baseline., Pneumococcal serotype specific colonisation in saliva, lymph node size in cortical thickness, long axis and short axis (determined by ultrasound), Comparing Spn PS-specific antibody titres in saliva to Spn PS-specific antibody titres in peripheral blood., Association between diet, excercise, alcohol consumption, smoking, comorbidities, medication, clinical Frailty score, CD4+ T cell count and immune response (% Bgc cells and Tfh cells, 4 weeks after vaccination)., Pneumococcal serotype specific colonisation in saliva and its association with post vaccination serotype specific B cells, serotype specific antibody response and OPA-titres in peripheral blood.

Related papers

No related papers found yet.

Interventions

DRUGPrevenar 20 suspension for injection in pre-filled syringe Pneumococcal polysaccharide conjugate vaccine (20-valent

DRUGadsorbed)

DRUGPNEUMOVAX 23 ενέσιμο διάλυμα σε προγεμισμένη σύριγγαΠολυσακχαριδικό Εμβόλιο Πνευμονιόκοκκου

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Design outcomes

Primary

Measure	Time frame
Immunophenotyping of lymph node FNA samples (% germinal centre B cells and %T follicular helper cells), 4 weeks after vaccination.	—

Secondary

Measure	Time frame
Immunophenotyping of PBMC's 6 months after vaccination (% S. pneumoniae PS-specific B cells per serotype, Spn PS-specific B cell phenotype, Spn PS-specific antibody titres, Spn PS-specific antibody fuctionality, CRM-197 specific B cells and T cells., Serum and lymph node supernatant biomarkers at day 7, day 28 and day 72 post vaccination, Immunophenotyping and functional analysis of PBMCs at baseline, Serum biomarkers at baseline., Pneumococcal serotype specific colonisation in saliva, lymph node size in cortical thickness, long axis and short axis (determined by ultrasound), Comparing Spn PS-specific antibody titres in saliva to Spn PS-specific antibody titres in peripheral blood., Association between diet, excercise, alcohol consumption, smoking, comorbidities, medication, clinical Frailty score, CD4+ T cell count and immune response (% Bgc cells and Tfh cells, 4 weeks after vaccination)., Pneumococcal serotype specific colonisation in saliva and its association with post vaccination	—

Measure

Time frame

Immunophenotyping of PBMC's 6 months after vaccination (% S. pneumoniae PS-specific B cells per serotype, Spn PS-specific B cell phenotype, Spn PS-specific antibody titres, Spn PS-specific antibody fuctionality, CRM-197 specific B cells and T cells., Serum and lymph node supernatant biomarkers at day 7, day 28 and day 72 post vaccination, Immunophenotyping and functional analysis of PBMCs at baseline, Serum biomarkers at baseline., Pneumococcal serotype specific colonisation in saliva, lymph node size in cortical thickness, long axis and short axis (determined by ultrasound), Comparing Spn PS-specific antibody titres in saliva to Spn PS-specific antibody titres in peripheral blood., Association between diet, excercise, alcohol consumption, smoking, comorbidities, medication, clinical Frailty score, CD4+ T cell count and immune response (% Bgc cells and Tfh cells, 4 weeks after vaccination)., Pneumococcal serotype specific colonisation in saliva and its association with post vaccination

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Countries

Netherlands

Outcome results

None listed