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Optimisation of lymph node fine needle aspiration to study pneumococcal vaccine-induced immunity

Status
Recruiting
Phases
Phase 2
Study type
Interventional
Source
EU CTIS
Registry ID
CTIS2022-501519-15-00
Enrollment
5
Registered
2023-02-02
Start date
2023-04-01
Completion date
Unknown
Last updated
2024-06-20

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy volunteers

Brief summary

Percentage of (polysaccharide-specific) germinal centre B cells in lymph node aspirates, as determined by spectral flow cytometry

Detailed description

Kinetics of the proportion of T follicular helper cells in lymph node aspirates, Dynamics in B cell and T cell phenotype in lymph node aspirates, including immunoglobulin class switching and activation/differentiation markers, Deep immunophenotyping of B cells and T cells in peripheral blood by spectral flow cytometry, Timing of seroconversion in peripheral blood, Cytokine concentration in peripheral blood and lymph node aspirate

Interventions

Sponsors

Leiden University Medical Center
Lead SponsorOTHER

Eligibility

Sex/Gender
All
Age
18 Years to 64 Years

Design outcomes

Primary

MeasureTime frame
Percentage of (polysaccharide-specific) germinal centre B cells in lymph node aspirates, as determined by spectral flow cytometry

Secondary

MeasureTime frame
Kinetics of the proportion of T follicular helper cells in lymph node aspirates, Dynamics in B cell and T cell phenotype in lymph node aspirates, including immunoglobulin class switching and activation/differentiation markers, Deep immunophenotyping of B cells and T cells in peripheral blood by spectral flow cytometry, Timing of seroconversion in peripheral blood, Cytokine concentration in peripheral blood and lymph node aspirate

Countries

Netherlands

Outcome results

None listed

Source: EU CTIS · Data processed: Feb 4, 2026