None listed
Conditions
Brief summary
Pathological production of polyols after brain injury may be a cause for secondary neurological injury. Polyols are osmotic and neurotoxic, which may have detrimental outcomes for critically ill individuals with brain injury. This study will be a biospecimen analysis of cerebrospinal fluid in health volunteers, aiming to quantify polyol concentrations. The results will act as a control group to compare with populations studied in other trials investigating polyol production in ICU patients with traumatic brain injury, subarachnoid haemorrhage, and pain conditions.
Interventions
Participants will have 4 mL of blood collected into a pre-chilled lithium heparin tube, and 4 mL of blood collected into a pre-chilled EDTA tube via venepuncture. Participants will have 15mL of CSF collected into a polypropylene tube, once only via aseptic lumbar puncture.
Blood glucose concentration will be measured concurrently using a portable bedside glucose meter (Optium Xceed: Abbott Laboratories, Bedford, MA).
A maximum of 16 mL of blood and 15 mL of CSF, will be collected from each parti
Participants will have 4 mL of blood collected into a pre-chilled lithium heparin tube, and 4 mL of blood collected into a pre-chilled EDTA tube via venepuncture. Participants will have 15mL of CSF collected into a polypropylene tube, once only via aseptic lumbar puncture. Blood glucose concentration will be measured concurrently using a portable bedside glucose meter (Optium Xceed: Abbott Laboratories, Bedford, MA). A maximum of 16 mL of blood and 15 mL of CSF, will be collected from each participant for this study. These biospecimens will be collected at a single time-point for each each participant, without any longitudinal observations. These biospecimens will be obtained from healthy volunteers (no acute brain pathology or metabolic disturbances) and will allow descriptions of brain glucose metabolism in health. Samples will be compared to cohorts with exposure to several conditions including: aneurysmal subarachnoid haemorrhage, traumatic brain injury, acute pain, chronic and neuropathic pain.
Sponsors
Central Adelaide Local Health Network
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
• Age greater than or equal to 18 years • Consenting to lumbar puncture procedure
Exclusion criteria
• Past medical history of diabetes mellitus or a screening HbA1C > 6.5% • Past medical history of traumatic brain injury • Past medical history of aneurysmal subarachnoid haemorrhage • Current smoker • Alcohol consumption >20g/day • Unable to safely tolerate lumbar puncture, including taking medicine where lumbar puncture is contraindicated (anti-coagulant besides 100 mg of aspirin) • Pregnancy • BMI > 30 kg/m2 • Surgery in preceding 6 months • Chronic pain • Migraines
Outcome results
None listed