None listed
Conditions
Brief summary
Amino acid sweat loss during high-intensity exercise in hot conditions may contribute to muscle fatigue and slower recovery after strenuous activity. The purpose of this project is to examine differences in post-exercise recovery, as measured by maximum voluntary isometric contraction (MVIC) of knee extensors and subjective fatigue rating, between intra-session HDAA supplementation and placebo. The aim is to examine the effect of HDAA supplementation against placebo in recreationally trained male and female cyclists. This study is a randomised, double-blind, crossover design involving two conditions: high-demand amino acid (HDAA) supplementation and a BCAA placebo control. Participants will be healthy recreationally trained cyclists 18-45 years of age. Objective and subjective measures of fatigue will be used to assess the effectiveness of the HDAA supplement compared to placebo in mitigating fatigue following strenuous exercise in the heat. Sweat will be collected and analysed for electrolyte concentrations during each exercise bout to examine relationships with electrolyte loss. Finally, muscle oxygenation will be used to examine the change in oxygenation patterns with supplementation.
Interventions
Supplement containing high demand amino acids -
Participants will consume 200mL of water with one recommended serve HDAA (ElectrAAte, InnovAAte, Newcastle, Australia) immediately prior to the exercise trial, with ad libitum consumption of room temperature water available to participants thereafter.
https://innovaate.com.au/products/optimaate?srsltid=AfmBOopGCN7GFkBF2hNKUIPlh2ndleswrFRUEXe_FwpRTMZt8OK7wX3S
Participants will complete a high-intensity interval cycling (HIIT) session on an electro
Supplement containing high demand amino acids - Participants will consume 200mL of water with one recommended serve HDAA (ElectrAAte, InnovAAte, Newcastle, Australia) immediately prior to the exercise trial, with ad libitum consumption of room temperature water available to participants thereafter. https://innovaate.com.au/products/optimaate?srsltid=AfmBOopGCN7GFkBF2hNKUIPlh2ndleswrFRUEXe_FwpRTMZt8OK7wX3S Participants will complete a high-intensity interval cycling (HIIT) session on an electronically braked cycle ergometer in a hyperthermic environmental chamber maintained at approximately 30°C. The session consists of six 4-minute work bouts at 110% of the second ventilatory threshold (VT2) power output, each separated by 3-minute active recovery periods at 50W. Total exercise time is 42 minutes (24 minutes of work + 18 minutes of recovery). Pre-visit adherence — Participant's will be provided with standardised guidance on pre-exercise nutrition, hydration, and exercise abstinence. Participants' compliance with pre-visit nutrition, hydration, and exercise abstinence requirements is verified through a checklist and urine collection and testing for specific gravity (USG < 1.020) and osmolality (< 700 mOsm/kg) on arrival. Direct observation of supplement consumption — Consumption of either the HDAA supplement or BCAA (active alternative treatment) is visually confirmed and recorded by the researcher present (either Dr Mitch Naughton or Ms Stephanie Graves) at the time of administration. Minimum of a 7 day washout between treatments (sessions)
Sponsors
The University of Newcastle
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Treatment
Masking
Blinded (masking used) (Subject, Caregiver)
Eligibility
Sex/Gender
All
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
- Adult male or female 18 – 45 years of age at the commencement of the study - Free from known medical conditions such as cardiovascular, metabolic, or renal disease or disorders which may be exacerbated by exercise and exposure to heat/dehydration (e.g., kidney disease, liver cirrhosis, diabetes mellitus). - Free from injury which would be exacerbated by exercise and dehydration - Low risk and active as assessed by the Exercise and Sports Science Australia Adult Pre-Exercise Screening System (APSS) - Able to freely provide saliva and urine samples - Free from known allergies to milk, wheat, eggs, soy, gluten or fish. - Can provide informed consent
Exclusion criteria
- < 18 years of age or >45 years of age at the commencement of the study - Have a known medical condition such as cardiovascular, metabolic, or renal disease or disorders which may be exacerbated by exercise and exposure to heat/dehydration (e.g., kidney disease, liver cirrhosis, diabetes mellitus). - At greater than low risk as assessed by the Exercise and Sports Science Australia Adult Pre-Exercise Screening System (APSS). - Have a current or reoccurring injury which would be exacerbated by exercise and dehydration - Known allergy to milk, wheat, eggs, soy, gluten, or fish. - Cannot provide or withdraws informed consent
Outcome results
None listed