The effect of bamboo bioferment as a support for the regeneration of skin damaged by microneedle mesotherapy in women.

Planned study group: The study will be conducted in a group of 60 women aged 40–55, who will be randomly assigned to one of four groups: 1) Group B control (15 people) – facial skin care with a cream base (daily application for 21 days) 2) Group BF (15 people) – facial skin care with a cream base with the addition of bamboo bioferment (daily application for 21 days) 3) Group MB (15 people) – microneedle mesotherapy of the facial skin + post-treatment care with a cream base (microneedle mesotherapy on day 1 with daily cream for 21 days) 4) Group MBF (15 people) – microneedle mesotherapy of the facial skin + post-treatment care with a cream base with the addition of bamboo bioferment (microneedle mesotherapy on day 1 with daily bamboo cream for 21 days) Planned studies: Facial care • The examined women - depending on the assignment to the study group - will undergo therapy consisting of performing facial skin care using a cream base, a cream base with the addition of bamboo bioferment and microneedle mesotherapy treatments. Each woman will be individually instructed on how to apply the base or base with bioferment. The instructions will include the application method and frequency. The women taking part in the study will apply the base or the base with bioferment for 21 days. The surveyed women will apply base and base with bioferment in their homes. Monitoring of compliance will be controlled through the use of patient diaries. o Basic care with a cream base - the basic cream base is a mixture of raw materials such as: water, emollient and a mild pH stabilizing substance, without the addition of active ingredients. The examined women will apply the cream base twice a day, in the morning and in the evening, massaging it into the skin of the face. A cosmetic spatula will be included for its application, allowing to measure the same amount of cream base with each application. o Care with a cream base with the addition of bamboo bioferment (cream base with the addition of 10% bamboo bioferment) - Similarly to the care with the cream base itself, here too the examined women will apply the base with the addition of bamboo bioferment twice a day, in the morning and in the evening, massaging it into the skin of the face. A cosmetic spatula will be included for the application of the cream, allowing to measure the same amount of cream with each application. o Microneedle mesotherapy treatment - a cosmetic treatment consisting in controlled puncturing of the skin with microneedles. This procedure will be performed once on each participant in the study. The treatment area will include the face (puncture depth 0.5 mm) and the eye area (puncture depth 0.25 mm). Each area will undergo 4 micro-puncture repetitions. The treatment will be performed by an experienced cosmetologist (the project's author). Each patient in the MB and MBF study groups will receive one treatment on the first day of the study. The treatment will last approximately 15 minutes. The treatment will be performed in the cosmetology laboratory on the university (University of Physical Culture in Krakow, Poland).