None listed
Conditions
Brief summary
Eating ultra-processed foods high in saturated fats and refined carbohydrates, and low in essential nutrients, can disrupt the body’s natural metabolic balance over time, leading to increased metabolic stress. This stress arises because energy-dense foods challenge the body’s ability to process fats and sugars. Previous studies have shown that consuming such meals can trigger a postprandial response, as indicated by a negative correlation between postprandial glucose area under the curve (AUC) and superoxide dismutase (SOD) levels both before and after eating. Incorporating more whole foods, such as fruits, into the daily diet may help mitigate the effects of less nutritious meals. Fruits are rich in essential nutrients that can alleviate oxidative stress and promote better metabolic health. Specifically, incorporating freeze-dried blueberries into a high-fat, high-carbohydrate meal may play a protective role against oxidative stress.. This study will assess whether incorporating fruits (blueberries) into a high-fat, high-carbohydrate meal could play a protective role against postprandial oxidative stress markers induced by the meal.
Interventions
This study is a double-blinded, 3-armed crossover, randomized postprandial trial, involving healthy male participants to make three visits to the testing facility. During each visit, participants will receive one of the following:
1. A high-fat high-carbohydrate milkshake without freeze-dried blueberries
2. A high-fat high-carbohydrate milkshake with a low dose of freeze-dried blueberries (15 g)
3. A high-fat high-carbohydrate milkshake with a high dose of freeze-dried blueberries (30 g)
On th
This study is a double-blinded, 3-armed crossover, randomized postprandial trial, involving healthy male participants to make three visits to the testing facility. During each visit, participants will receive one of the following: 1. A high-fat high-carbohydrate milkshake without freeze-dried blueberries 2. A high-fat high-carbohydrate milkshake with a low dose of freeze-dried blueberries (15 g) 3. A high-fat high-carbohydrate milkshake with a high dose of freeze-dried blueberries (30 g) On the testing visits, the participants will attend the testing facility from 9.00 am to 5.00 pm. Participants will arrive to this visit following an overnight fast after consuming regular dinner between 7.00 and 9.00 pm. Participants will also be asked to maintain their regular diet and avoid strenuous exercise for the 24 hours before the beginning of the testing session. Participants will receive reminders via text message prior to the testing session to remind them of these requirements. During the first testing visit, participants will complete a 24-hour food recall, and have their height, weight, waist circumference, body composition, and blood pressure measured. For all three testing visits, a cannula will be inserted into a vein in the participant's arm by a nurse or trained researcher for multiple blood draws. Blood samples will be collected during the 2 hours before and 6 hours following meal consumption. Blood samples will be collected every 60 minutes during the 2 hours before food consumption. Following a two-hour run-in period, including venous blood collection, participants are given a high-fat high-carbohydrate meal in the form of a milkshake to consume within 10 minutes (time 0). The milkshakes will contain either no blueberries, a low dose, or a high dose of freeze-dried blueberries, with commercial artificial blueberry flavoring added to maintain blinding. Adherence will be verified by recording the consumption time and through visual confirmation of the milkshake container by the independent researcher who manages the blinding. Following this, venous blood samples will be collected every 15 minutes for the first hour, every 30 minutes for the following two hours, and then every 60 minutes up to 6 hours following the meal. All blood samples will be taken by a nurse or by researchers trained in phlebotomy.
Sponsors
Monash University
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Prevention
Masking
Blinded (masking used) (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
Male
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
Adult men aged between 18 and 35 years Body mass index between the range of 18.5 to 29.9 kg/m2 Non smoker and non vaper
Exclusion criteria
- Body mass index outside the range of 18.5 to 29.9 kg/m2 - Aged less than 18 or greater than 35 years - Smoker and vaper - Currently have chronic medical condition, such as gstrointestinal disease or autoimmune disease - Receiving regular medication - Dietary allergies or restrictions that prevent consuming the meals, i.e. lactose intolerant or allergies to the milkshake ingredients (milk, blueberries)
Outcome results
None listed