In vitro study of the effects of sugammadex on the measurement of serum concentrations of progesterone in premenopausal women – an assay validation study

Single blood sample (20mL) collected using routine blood collection techniques from premenopausal women. Then testing if sugammadex interacts with a progesterone assay at various sugammadex concentrations. Serum samples will be centrifuged and spiked with various sugammadex concentrations. Serum sample divided into 7 volumes and spiked with: 10, 40 , 80, 160, 480, 600, 1200, 4800 microM sugammadex concentrations The assay being utilised is Elecsys Progesterone III The only involvement by participiants is the provision of blood An additional measurement technique will be used in our study. This element will involve further testing of our progesterone samples using a non-immunoassay method. This will allow us to compare progesterone samples with different measurement techniques and observe any differences. This second part of our study will not require any further blood taking or intervention on the part of participants. Additionally consent has already been gained earlier in the study. Ethics approval has already been update and granted for this aspect of our study. When the changes were implemented: After recruitment was completed