None listed
Conditions
Brief summary
This study will help us assess the effect of a high-fat meal on our blood vessels (cardiovascular health) and how naturally occurring polyphenolic compounds in plant foods could potentially affect this beneficially. An important marker of cardiovascular health is the flexibility of the blood vessels such as arteries, their ability to dilate and constrict when needed. Endothelial function can be measured using a non- invasive technique called “flow mediated dilatation” abbreviated to FMD. The health and function of an artery can be assessed by measuring someone’s FMD in the morning, after fasting overnight. Poor diet can adversely affect artery health. There is evidence that consumption of excess dietary fat contributes towards the dysfunction of our arteries. Consumption of a single, high fat meal impairs post-meal FMD and the typical western diet is characterised by a high frequency of high-fat content meals. Polyphenols are micronutrients mainly found in plant-based foods. Recently, clinical and experimental trials have focused on exploring whether polyphenols can have a beneficial effect on health. Polyphenols can protect blood vessels due to their antioxidant properties, acting directly on the endothelium. The researchers will assess how foods rich in polyphenols (blueberries and cocoa) could play a protective role in the arteries during a high-fat meal challenge by measuring FMD.
Interventions
This study will help us assess the effect of a high-fat meal on our blood vessels (cardiovascular health) and how naturally occurring polyphenolic compounds in plant foods could potentially affect this beneficially. This will be a single-blinded, 3-armed randomised crossover interventional trial in young, apparently healthy men.
Participants will be screened via an online survey for eligibility with likely candidates invited for an in-person screening session at the BASE facility (Be Active Sl
This study will help us assess the effect of a high-fat meal on our blood vessels (cardiovascular health) and how naturally occurring polyphenolic compounds in plant foods could potentially affect this beneficially. This will be a single-blinded, 3-armed randomised crossover interventional trial in young, apparently healthy men. Participants will be screened via an online survey for eligibility with likely candidates invited for an in-person screening session at the BASE facility (Be Active Sleep Eat) at Monash University, Notting Hill campus. At the screening visit, anthropometry, blood pressure, endothelial function via flow mediated dilation (FMD) and fasting glucose, triglycerides and cholesterol will be measured by a trained researcher. After completion of a physical activity questionnaire, a general demographics survey and a food record participants will be booked in for a testing visit. Each participant will complete arm 1 and 2 testing visits plus can opt-in for the third arm testing visit. Arm 1: High-Fat milkshake + control (1g fat per kilo of bodyweight) Arm 2: High-Fat milkshake + 26 g freeze dried blueberries + 12.24g high flavanol cocoa powder (per 75 kg of bodyweight) Arm 3: Fasting All interventions will be delivered face-to-face on at least two separate occasions over 7.5 hours. There is a minimum 1 week washout period between the intervention sessions. For 24 hours before testing, participants will avoid strenuous exercise and consume at least 2 L of fluids. On the morning of testing, following an overnight fast from 9 pm (water is allowed) participants will present at BASE and undergo baseline measurements, participants will then either consume the high fat meal plus additives or continue to fast. All intervention food will be consumed under supervision. After meal consumption, venous blood samples will be collected every 15 minutes for the first hour, every 30 minutes for the following two hours, and then every 60 minutes up to 6 hours following the meal. FMD and blood pressure will be measured every hour post-meal.
Sponsors
Gary Williamson
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Prevention
Masking
Blinded (masking used) (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
Male
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
Young (More than 18 years old and less than 31 years old), apparently healthy, non-smoking males with a BMI between 18.5 or above 27 kg/m2.
Exclusion criteria
- Less than 18 years of age or older than 31 years of age - Body mass index (BMI) less than 18.5 or above 27 kg/m2 - Be a current smoker or have ceased smoking within the past six months - Biological sex is female - Individuals who have an arteriovenous fistula or implantable cardiac defibrillator/cardiac pacemaker - Fasting triglycerides above 1.7 mmol/L and fasting glucose above 7 mmol/L - Blood pressure below 90/60 or above 140/90 mmHg - A confirmed diagnosis of T2D or a diagnosis of lipid-related disorder (e.g hypercholesterolemia, fatty liver); anyone on lipid lowering medication or other medications. - People who do not wish to have blood samples collected or cannot tolerate the sight of blood - Individuals with dietary allergies that prevent them from consuming study meals - People who cannot read or write English
Outcome results
None listed