Histological analysis and imaging of explanted pelvic mesh

Histological, immunochemistry and imaging of explanted gynaecological mesh in women with or without mesh complications All patients who are seen as part of urogynaecology outpatinets and consented for removal of mesh are invited to participate in the study. Samples will be taken as part of routine care for the patient - for those with mesh complications, mesh will be removed. For those that are having subsequent procedures for management of incontinence/prolapse and have had previous mesh procedures, the mesh will be removed from the operating area to allow the subsequent procedure to proceed. Histological analysis will involve hematoxylin and eosin staining, tissue morphology, identification of foreign body giant cells, polymorphonuclear cells and blood vessels. Immunohistocehmistry will look at macrophage presence, neovascularisation and specific inflammatory reactions. Electron microscopy will be performed to investigate microstructure differences in explanted mesh samples Routine follow-up of each patient will be 6 weeks post op check up and 6 month review as per the recommendations from the Australian Commission on Safety and Quality in Healthcare in patients who have had mesh removal (this is routine post surgical follow-up and occurs for all patients regardless of entry into the trial).