None listed
Conditions
Brief summary
Autophagy is a critical process that helps keep the body’s cells clean and healthy. It is thought to be sensitive to nutrition. Using a test that we have developed to measure autophagic activity in humans for the first time, this project aims to determine whether autophagic activity in the blood is changed after healthy study participants have consumed a drink containing 30 g of whey protein compared to a fasted state. Our hypothesis is that autophagy is inhibited after a protein-rich drink in fasting healthy humans. Target sample size is 40, however HREC approval obtained to recruit between 40-60 participants as necessary.
Interventions
This study is a single-arm pre-post study that aims to measure the effect of a protein-rich breakfast on autophagic flux (i.e. a cell mechanism that cleanses the cells) in fasting healthy individuals.
After an overnight fast (12 hours, with permission to drink water), participants will attend a 1.5 hours intervention appointment at the clinical facility of the South Australian Health and Medical Research Institute for testing. Participants will be asked to refrain from strenuous exercise and alc
This study is a single-arm pre-post study that aims to measure the effect of a protein-rich breakfast on autophagic flux (i.e. a cell mechanism that cleanses the cells) in fasting healthy individuals. After an overnight fast (12 hours, with permission to drink water), participants will attend a 1.5 hours intervention appointment at the clinical facility of the South Australian Health and Medical Research Institute for testing. Participants will be asked to refrain from strenuous exercise and alcohol the day before the test. They will arrive at 8 a.m. After collection of anthropometric information (weight, height, waist and hip circumference and blood pressure), a fasting blood sample will be drawn. Immediately after the blood collection, participants will be asked to consume a standardised whey protein isolate powder (30 g, 90% protein) diluted in skim milk (250 mL) in less than 5 min. The drink will be prepared and provided by a member of the clinical study research team, who will monitor that the drink is consumed entirely within the allocated 5 minutes. After a resting period of 60 min post drink consumption, a second blood collection will be performed. During the resting period, participants will be permitted water (but no other food or drinks) and will be asked to remain sedentary. Autophagic flux will be measured in the blood samples obtained from participants before and after consumption of the protein-rich drink.
Sponsors
Dr Timothy Sargeant
Study design
Allocation
Non-randomised trial
Intervention model
Single group
Primary purpose
Prevention
Masking
Open (masking not used)
Eligibility
Sex/Gender
All
Age
20 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
1. Aged between 20 and 50 years of age 2. BMI between 18.5 and 29.9 kg/m2
Exclusion criteria
1. Any co-morbidities that are likely to change the activity of the lysosomal system, such as cancer, diabetes, cardiovascular disease/conditions (stroke, heart attack, high blood pressure), major psychiatric disorders (schizophrenia, addiction, eating disorders, major depressive disorder), neurological disorders, dementia, and/or any other condition deemed likely to affect the results by the study principal investigator. 2. Taking any medications that, in the opinion of the investigator, might change autophagic activity; including, but not restricted to, medications that change body composition or metabolism (e.g. medications used to lower blood glucose, antidiabetic medications), anti-inflammatory medications, medications that change brain function (e.g. antidepressant medications, mood stabilisers, lithium), medications used in the treatment of cancer or cardiovascular disorders. Female participants taking hormonal contraceptive will not be excluded from the study. If they have a regular menstrual cycle, we will ask that they attend the study appointment between day 1 and day 10 of their menstrual cycle. 3. Current alcohol and/or substance use disorder. 4. Lactose intolerance (except if managed by medication eg. Lacteeze). 5. Vegan. 6. Allergies preventing consumption of study product. 7. Current smoker. 8. Pregnant or breastfeeding women or women planning a pregnancy. 9. Women who have been through menopause. 10. Any lifestyle (e.g. physical activity, dietary habits) and/or change in lifestyle deemed likely to affect the results by the study principal investigator. 11. Anyone who is unable to comprehend the study protocol (i.e. due to English language or cognitive difficulties).
Outcome results
None listed