Health benefits of Vitamin C supplementation during health training on physical fitness, body composition and genes expression in elderly women

In this study the effect of health training (2x 6-week periods, three times per week for 60 minutes in duration) and suplementation vitamin C in dose 1000 mg/day will be investigation. Vitamin C will be administered in tablets. All participant will keep a supplementation diary. Physical effort and antioxidant supplementation may influence on training adaptation. Vitamin C is well known as antioxidative Vitamin and through reduction in oxidative stress can influence on genes expression, especially easy induce by exercise. Our study will be involve 15 elderly women (a mean age of 69.2 ± 6.5 years ) randomly chosen to a cross-over study. Women will be randomly divide into two groups. At first 6-week period of health training 7 of them will be supplemented Vit. C while 8 of them will receive a placebo (fiber tablets). In the second 6 week 8 of them (earlier received placebo) will be supplemented and trained while 7 of them will receive fiber in tabs. The wash out period (the break in sessions will last a week). Each volunteer will be qualified by a physician before participation in the study. The health training will be supervised by the members of our research team. consisting of water gymnastics, gyrokinesis, stabilization training, and Nordic walking at moderate intensity. The type of exercise will be planned in advance for the entire period so that the individual classes are equally distributed. The trainer will additionally record the implementation of the training plan. The training will be conducted for all participants simultaneously, in one group of 15 people. During training, participants maintained their heart rate (Polar H1) at less than 130/min. During whole experiment participants will sign session attendance checklists. All of the tests will be performer in Medical University in Gdansk (genes expression analysis) and University of Physical Education in Kraków (biochemical analysis and level of Vitamin C in serum). Before and after the first and last interventions period (first and second 6 weeks intervention) measurements of anthropometric indices, body composition are performed. Venous blood is collected from the volunteers four Times: before the start of the experiment, after 6 weeks, before start second 6 week period and at the end of experiment. Vitamin C analysis will be performer immediately after collection, for biochemical analysis serum or plasma will be separate and stored in -80oC, for genetic research lysed leukocytes will be stored in -20oC for further analysis.