None listed
Conditions
Brief summary
Anastomotic leakage (AL) is amongst the most significant complications of intestinal surgery and results in increased morbidity and mortality. Recent research has shown that certain species of E. coli and Pseudomonas have been associated with AL in colorectal anastomoses. These organisms produce collagenases and metalloproteinases that can break down a newly constructed and healing anastomosis. Much research has also gone into defining bacterial populations that are associated with pouchitis. Our project aims to describe the microbiological communities in the terminal ileum and rectum that will contribute to the ultimate ileal pouch microbiome. The future application of this information is to allow intervention for manipulation of the microbiome to more favourable microbial profiles prior to surgery, and thus reduce the leak rate and perhaps, in the long term, protect against the development of pouchitis and perianal disease.
Interventions
After written consent is obtained:
- Day of surgery (Day 0): stool sample (0.5 ml) will be collected from ileostomy and tissue biopsy will be collected from the resected ileum and rectum. Samples will be immediately frozen on dry ice and stored at -80oC until microbiome analysis.
- Clinical course observation during admission to establish participants who experienced anastomotic leak and participants who did not compare anastomotic leak
Sponsors
Royal Brisbane Womens Hospital (RBWH)
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
Ileal pouch surgery without a covering loop ileostomy J, W or S pouch configuration Indication must be Ulcerative Colitis Must be staged (ie. prior total colectomy and ileostomy)
Exclusion criteria
Age less than 18 years Inability to give consent Pregnant
Outcome results
None listed