None listed
Conditions
Brief summary
Background: Eosinophilic esophagitis (EoE) and gastroesophageal reflux disease (GERD) can be difficult to distinguish as many of their clinical and histological features overlap. Preliminary data suggests a potential association between EoE and immunoglobulin G4 (IgG4), but not GERD. Aim: To examine the role of esophageal mucosal IgG4 staining in differentiating EoE from GERD. Methods: Esophageal biopsy specimens from patients with proven EoE and GERD were evaluated and immunohistochemical staining for IgG4 was performed by an experienced gastrointestinal pathologist blinded from the clinical and endoscopic data. The results on IgG4 staining was then correlated with clinical, endoscopic and histological features.
Interventions
IgG4 staining was performed prospectively on retrospective specimens. Thus, the ethics committee approved the study to proceed without requiring patient consent. Medical records were accessed as approved by the ethics committee.
This was a single cross-sectional assessment.
All biopsies with either EoE or GORD will undergo immunohistochemical staining for IgG4 using DAKO Autostainer Plus Staining System. A single pathologist who specializes in gastrointestinal pathology will perform all immunohi
IgG4 staining was performed prospectively on retrospective specimens. Thus, the ethics committee approved the study to proceed without requiring patient consent. Medical records were accessed as approved by the ethics committee. This was a single cross-sectional assessment. All biopsies with either EoE or GORD will undergo immunohistochemical staining for IgG4 using DAKO Autostainer Plus Staining System. A single pathologist who specializes in gastrointestinal pathology will perform all immunohistochemical staining and interpretation of the slides in a blinded manner.
Sponsors
GI and Pancreatic Researchs Group
Eligibility
Sex/Gender
All
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
GERD: -18-80 years of age -typical symptoms of GERD responsive to PPI therapy -evidence of oesophagitis on endoscopy with supportive oesophageal biopsy specimens --eosinophil count <10/hpf EoE: -18-80 years of age, symptoms of oesophageal dysfunction with 'greater than or equal to' 15 eosinophils/hpf -exclusion of other causes of eosinophilia
Exclusion criteria
Exclusion criteria were history of severe respiratory, cardiovascular, hepatic, haematological and/or renal disease, chronic alcohol abuse, medications that may influence gastrointestinal function and previous gastrointestinal surgery.
Outcome results
None listed