None listed
Conditions
Brief summary
The current project proposes to collect human faecal samples from healthy individuals, and use them to create an in vitro (ex-vivo) model of the colon.Participants will be asked to provide a breath sample to be analysed for methane concentration, and particpants will be divided into 2 groups; methane excretors and non-methane excretors. Different dietary fibres will be added to the model, and the characteristics of fermentation will be monitored. The main hypothesis is that the fermentation of dietary fibre will differ according to methane breath excretion by the faecal donor. This can help elucidate some of the individual differences between people that affect beneficial dietary fibre fermentation in the human colon.
Interventions
1. Health screening questionnaire - single session completed via email, takes approximately 10 minutes
2. Collection of breath sample for analysis of breath gases, single 20 minute session (occurring Dec-Jan 2019/2020. Approx 2-4 weeks after health screening questionnaire).
3. 3 day food and hydration diary, 10 minutes per day for 3 consecutive days immediately prior to faecal sample donation (approx 1-10 weeks after breath sample)
4. Collection of faecal sample used to seed ex-vivo (in-vitro)
1. Health screening questionnaire - single session completed via email, takes approximately 10 minutes 2. Collection of breath sample for analysis of breath gases, single 20 minute session (occurring Dec-Jan 2019/2020. Approx 2-4 weeks after health screening questionnaire). 3. 3 day food and hydration diary, 10 minutes per day for 3 consecutive days immediately prior to faecal sample donation (approx 1-10 weeks after breath sample) 4. Collection of faecal sample used to seed ex-vivo (in-vitro) fermentation laboratory model, single 20 minute session (occurring Jan-Feb 2020 i.e. 1-10 weeks after initial breath sample) Note: All breath samples will be taken from all participants in a 2-3 week timeframe. After all breath samples have been processed, participants will be asked to provide faecal samples (1-2 person per week) for the following 8 weeks. Faecal samples must be used fresh for the ex-vivo work, and the laboratory setup for the experiment takes approximately 1 week.
Sponsors
Riddet Institute
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Medically healthy (according to exclusion criteria listed below) BMI between 18.5 and 30 Long term consumption of a moderate-high fibre diet (at least 15 g/day) Happy to provide a 3-day food and hydration diary Happy to complete a general health screening questionnaire
Exclusion criteria
According to a health screening questionnaire: Some current health concerns Previously diagnosed with norovirus, hepatitis A or intestinal parasite (ever) Currently experiencing any flu-like symptoms (fever, headache, dehydration, weight loss, lethargy) Noticed blood in faeces over the last 3 months Previously diagnosed with Salmonella, Shigella, Yersinia, Campylobacter, Aeromonas, Candida, E.coli, Klebsiella, Cryptosporidium, Entamoeba histolytics (ever) Somebody in the household has had diarrhoea, vomiting, stomach pain or upset stomach in the last 3 months Experienced gastrointestinal symptoms over the last 3 months (loss of appetite, nausea, vomiting, diarrhoea) Taken medicines over the last 2 weeks excluding contraception Used antibiotics or laxatives in the last 3 months According to the Bristol Stool Chart, most common type of stool is rated 1 or 7 In a typical day, defecate less than 1 time per day
Outcome results
None listed