Survival of autologous fat transfer (AFT) for scar remodeling with and without platelet rich plasma (PRP)

In group A, Fat Graft with PRP will be used. The fat will be harvested by consultant Plastic Surgeon from medial thigh. The procedure will done under local anesthesia. The duration of procedure will be around 60 minutes. The procedure will be done to correct the contour deformity of the patients by filling the fat in different layers of skin and underlying tissue in single procedure. Tumescent or infiltration Solution was made of 0.5% lidocaine with 1:200000 epinephrine diluted in 200 ml lactate ringer solution. This was injected with 17 G cannula in subcutaneous tissue of donor area. The injection and harvesting was done at 2:1. The fat with 23G cannula was harvested and prepared with the help of gravity. The fat separated from fluid and oil. The PRP will be obtained from the blood of the patient, 20 ml of blood will be obtained by the technician at the start of the procedure before harvesting the fat. The blood will be centrifuge at 1500 revolutions for 5 minutes and then at 2500 revolutions for 10 minutes. The plasma will be separated. 5 cc of adipocytes + 3 ml of PRP in 10 ml syringes with adding 10% CaCl (0.05 cm3 of CaCl per 3 ml). The extracted fat will be injected with 1cc syringe attached to 14 G needle in multiple layers. 20-30% over correction of contour deformity was done because of chance of absorption. At the end of the procedure, the steri strip over the recipient and dressing for donor sites was applied.