Efficacy of nanosilver and chlorhexidine in the treatment of plaque-induced gingivitis

This study is a triple-blinded randomized controlled comparative trial of four weeks duration investigating the efficacy of a locally available nanosilver and chlorhexidine mouth wash in the treatment of plaque-induced gingivitis. Subjects received a dental prophylaxis in the past two weeks prior to the baseline examination. Enrolled subjects were then randomly divided into two groups: (1) Control group who rinsed with 0.12% CHX Chlorhexidine Glucognate mouthwash utilizing 10 mL twice daily for one minute. (2) Experimental group who rinsed with less than 0.01% NS Nanosilver mouthwash utilizing 10 mL twice daily for one minute. All patients received a complete dental prophylaxis to remove all plaque, calculus and extrinsic stain before entering the study. The participants were motivated on regular intervals by personal and phone contact to use soft tooth brush, brush only with similar toothpaste (Colgate) and to brush their teeth twice daily, once in the morning after breakfast and once in the evening before bedtime. They were instructed to brush a minimum of 3 minutes to ensure thorough brushing. They also were informed about the same brushing technique (bass technique). They were instructed to rinse their mouth with mouth wash at least half an hour after tooth brushing and instructed not to take any liquid o food for at least 30 minutes. The subjects were instructed not to take any liquid or food into the mouth for at least 30 minutes after using mouth wash to avoid reduction the substantively of mouth wash, thus decreasing the efficacy of the mouth wash and to diminish the side effects of CHX like staining and bad taste. Then we contacted the patients every day at the morning and evening during a period of study by missed calls and message text to remind them. After one week from rinse use the patients returned the empty bottles to receive another one and delivery of the patient diary supplied by the researcher.