None listed
Conditions
Brief summary
There is not enough data in the medical literature to distinguish whether a systemic inflammatory response is caused by an infectious agent or aseptic inflammation due to acute coronary syndrome, especially during the perioperative period. Attention is drawn to microRNAs whose expression changes in patients with the infectious disease. The study will include 100 patients older than 18 years, who have the abdominal sepsis (peritonitis) and are going abdominal surgery intervention, patients with acute cardiovascular syndrome and high inflammatory biomarkers, patients without inflammation, ischemic heart disease who are who are going for minor elective surgery. All patients will be provided with complete study information before being asked to provide consent. We will collect data describing demographics, severity of illness (SOFA; APACHEII scores). Blood samples will be collected at the time of other standard-care assessments to minimize/eliminate the need for additional punctures in the first 24 hours of hospitalization. Up to 30 ml of blood will be collected for measuring the levels of biomarkers expression. Follow up will occur until the end of active surveillance, death or hospitalization period. Blood samples will be taken within the first 24 hours after hospitalization. The expression of microRNAs will be assessed in patients with different severity of abdominal sepsis, acute cardiovascular syndrome with high inflammatory biomarkers and control patients without cardiovascular dysfunction or inflammation. RNA extraction kits will be using for extracting microRNA from blood serum. Levels of microRNA's expression will be assessed with PCR. We will assess differential value of microRNAs in patients with different severity of abdominal sepsis and acute cardiovascular syndrome with high inflammatory biomarkers.
Interventions
We will involve patients with abdominal sepsis, who are going surgery intervention, patients with acute coronary syndrome and patients without inflammation,non infection SIRS, ischemic heart disease, who are going minor elective surgery. We will collect data describing demographics, severity of illness (SOFA; APACHE II scores). Blood samples will be collected at the time of other standard-care assessments to minimize/eliminate the need for additional punctures in the first 24 hours of hospitali
We will involve patients with abdominal sepsis, who are going surgery intervention, patients with acute coronary syndrome and patients without inflammation,non infection SIRS, ischemic heart disease, who are going minor elective surgery. We will collect data describing demographics, severity of illness (SOFA; APACHE II scores). Blood samples will be collected at the time of other standard-care assessments to minimize/eliminate the need for additional punctures in the first 24 hours of hospitalization. Blood samples from patients, who are going minor elective surgery, will be taken during venous puncture before the surgery intervention. Up to 30 ml of blood will be collected for measuring the levels of biomarkers expression. Follow up will occur within first 48 hours, death or hospitalization period up to 60 days.
Sponsors
Andrius Macas
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Patients with abdominal sepsis (peritonitis) who undergo abdominal surgery, Patients with acute cardiovascular syndrome and high inflammatory biomarkers. Patients without inflammation,non infection SIRS, ischemic heart disease.
Exclusion criteria
Oncological disease, pegnancy, refusal for participation, prisoners
Outcome results
None listed