None listed
Conditions
Brief summary
This study aims to look at whether the complex lipids in beef are absorbed and stored by the body, and whether this has an effect on biomarkers of cardiovascular disease risk. We will compare diets including either (1) NZ grassfed Wagyu beef, (2) grainfed commercial beef and (3) vegetable-based meat alternatives. Overweight/obese middle aged men with dyslipidaemia will be included as they represent a major consumer group of red meat who are also at a greater risk of developing cardiovascular disease later in life. This study will assess cardiovascular and metabolic health before and after 8 weeks of a diet including red meat or meat alternatives. This will be achieved by looking at blood and tissue levels of complex lipids, the impacts on cholesterol (amount and size) and overall metabolism, and the effects on the microbiome and its metabolic activity.
Interventions
Subjects will consume 3 times weekly for 8 weeks standard servings (167g) of either:
(1) NZ grass-fed Wagyu beef
(2) grain-fed commercial beef or
(3) vegetable-based meat alternatives
Adherence to the study protocol will monitored through questionnaires filled out in front of study personnel during fortnightly visits to the Liggins Institute.
An overnight fast (10 hours) is required of the participant immediately before the baseline visit and the post-intervention visit, including all food and d
Subjects will consume 3 times weekly for 8 weeks standard servings (167g) of either: (1) NZ grass-fed Wagyu beef (2) grain-fed commercial beef or (3) vegetable-based meat alternatives Adherence to the study protocol will monitored through questionnaires filled out in front of study personnel during fortnightly visits to the Liggins Institute. An overnight fast (10 hours) is required of the participant immediately before the baseline visit and the post-intervention visit, including all food and drink although water is permitted. Adherence to fasting is assured by food recall and subject confirmation.
Sponsors
AgResearch Limited
Study design
Allocation
Randomised controlled trial
Intervention model
Parallel
Primary purpose
Treatment
Masking
Blinded (masking used) (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
Male
Age
35 Years to 55 Years
Healthy volunteers
No
Inclusion criteria
Subjects will be: • Male • 35-55 years • BMI 25-35 kg/m2 • Total non-fasting cholesterol >5.0 mmol/L and/or LDL-C >3.0 mmol/L and/or HDL-C <1.0 mmol/L, and/or non-fasting triacylglycerides >2.0 mmol/L • No history of heart disease, gastrointestinal disease, or metabolic disease
Exclusion criteria
Participants will be excluded from participation if they: • Have heart, metabolic, or gastrointestinal disease • Random blood glucose greater than 11.1 mmol/L • Are currently taking medications for cardiovascular disease, or which are expected to interfere with normal digestive or metabolic processes including proton pump inhibitors, laxatives, etc. - Have taken antibiotics within the preceding 3 months • Have a medical history precluding a healthy state: history of myocardial infarction, angina, stroke, cancer or pre-existing diabetes, self-reported alcohol intake exceeding a moderate intake (>28 units per week) • Are smokers
Outcome results
None listed