None listed
Conditions
Brief summary
Aim: To evaluate the anti-inflammatory, antioxidant and bioavailability of polyphenols in obese populations post-coloured rice consumption. Design/Participants: The anti-inflammatory, antioxidant properties and polyphenol bioavailability will be tested by collecting fasting and post rice consumption blood samples from healthy but sedentary obese (perform < 3 hours of exercise weekly, BMI > 30) participants. Methodology: Visit 1: Health screening and questionnaires (recruitment). Visit 2: Fasting volunteers will be cannulated and baseline blood samples (25 mL) will be obtained. Volunteers will then be fed a serving of cooked coloured rice 1 (200 g or one cup). Post consumption, blood samples will be collected at different time points (30 min, 1h, 2h, 4h)a second blood sample (25 mL) will be taken and analysed. Visit 3 & 4: Crossover for coloured rice 2 and coloured rice 3 respectively. All blood samples will be analysed for Aanti-inflammatory properties will be evaluated for( pro-inflammatory cytokines and lipid peroxidation); antioxidant properties will be evaluated using( total antioxidant assays) and bioavailability of polyphenols will be determined by( HPLC). Expected outcomes: Consumption of coloured rice rich in polyphenols might potentially alleviate biomarkers of inflammation and oxidative stress in obese population.
Interventions
1. Participants in a fasting state (minimum 8 hours) will be prepared for cannulation. An intravenous cannula will be inserted into the arm vein using standard cannulation procedures (Guideline for PIVC Insertion and Post Insertion Care in Adult Patients, NSW Health) by a trained phlebotomist.
2. Baseline blood samples (25 mL) will be obtained and the participants will consume a serving of plain cooked coloured rice (one cup or 200 g) in week 1 (day 1) - black, red or brown rice (randomised)
3.
1. Participants in a fasting state (minimum 8 hours) will be prepared for cannulation. An intravenous cannula will be inserted into the arm vein using standard cannulation procedures (Guideline for PIVC Insertion and Post Insertion Care in Adult Patients, NSW Health) by a trained phlebotomist. 2. Baseline blood samples (25 mL) will be obtained and the participants will consume a serving of plain cooked coloured rice (one cup or 200 g) in week 1 (day 1) - black, red or brown rice (randomised) 3. After rice consumption, blood samples (25 mL at each time point) will be taken at regular time intervals 0.5, 1, 2 ,4 ,6 hours post rice consumption. 4. The cannula will be removed and the site covered with sterile gauze. 5. One week of wash out between cross over 2 (week 2 - day 7) and 3 (week 3 - day 14) will be applied.
Sponsors
Dr Abishek Santhakumar
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Treatment
Masking
Open (masking not used)
Eligibility
Sex/Gender
All
Age
18 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
• Obese (BMI> 30) • Non-smoker • Caucasian • Performs less than 3hrs exercise per week • Male or Female (18 - 65 years) • No current or medical history of Chronic diseases • Non-pregnant • Not currently taking anti-inflammatory, anti-dyslipidaemic or anti-clotting medications • No known problems with venepuncture • Not currently taking multivitamin supplements
Exclusion criteria
• Normal BMI • Smoker • Non-Caucasian • Performs more than 3hrs exercise per week • Male or Female (less than 18 years or greater than 65 years of age) • Currently suffering from chronic disorders • Pregnant • Currently taking anti-inflammatory, anti-dyslipidaemic, anti-clotting medications or multivitamin supplementations
Outcome results
None listed