None listed
Conditions
Brief summary
This randomised, within subject cross-over pilot study aims to quantify the acute dose-dependent changes in intact anthocyanins (such as cyanidin-3-rutinoside, cyanidin-3-glucoside, peonidin-3-rutinoside and pelargonin-3-rutinoside) and phenolic acid metabolites (such as vanillic acid, hippuric acid, ferulic acid) in biological specimens (urine, faecal matter) over 24 hours after consumption of 200 g and 500 g anthocyanin-rich Tasmanian sweet cherries (‘Lapins’). It is hypothesised that anthocyanin-rich Tasmanian sweet cherry consumption will increase anthocyanin and phenolic acid metabolite biomarkers in biological samples (urine, faecal matter) in a dose-dependent manner. This is a preliminary and fundamental step which will be useful in future studies, as a measure of compliance to the intervention, and may provide evidence of the mechanisms of action by which cherry anthocyanins are perceived to work.
Interventions
Sweet ‘Lapins’ cherries were harvested in January 2018, washed, de-stemmed, pitted, vacuum-packed in portions of 200 g or 500 g and stored at -20 °C to minimise anthocyanin degradation. The cherries are first-grade fruit, that were picked from a commercial orchid.
a) the dose administered in each treatment:
arm 1: 500g cherries, arm 2: 200g cherries
b) the frequency/duration of administration
Participant will consume cherries once off in each arm
c) the mode of administration
Cherries will be
Sweet ‘Lapins’ cherries were harvested in January 2018, washed, de-stemmed, pitted, vacuum-packed in portions of 200 g or 500 g and stored at -20 °C to minimise anthocyanin degradation. The cherries are first-grade fruit, that were picked from a commercial orchid. a) the dose administered in each treatment: arm 1: 500g cherries, arm 2: 200g cherries b) the frequency/duration of administration Participant will consume cherries once off in each arm c) the mode of administration Cherries will be eaten whole, with a standardised breakfast low in flavonoids (wheat biscuits, milk) d) the duration of 'washout' between the crossed-over treatments There will be a two-week washout between intervention arms e) any strategies used to monitor adherence to the intervention The research team will use direct observation to monitor adherence to the intervention
Sponsors
University of Tasmania
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Diagnosis
Masking
Open (masking not used)
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Eligible participants (healthy adults, male/female, aged 18y+) are those who do not have allergies to anthocyanin-containing fruit and vegetables (including sweet cherry) or standardised, low-flavonoid food provided on the study days, those who do not have diseases of the gastrointestinal tract (e.g. Crohn’s disease – which may affect metabolism of food), and those who do not take flavonoid-containing dietary supplements (as this may confound the metabolites).
Exclusion criteria
Exclusion criteria include allergies to specified foods, diseases of the gastrointestinal tract, consumption of flavonoid-containing dietary supplements, pregnancy and/or breastfeeding (as this may affect metabolism), and an inability to provide informed consent.
Outcome results
None listed