Detection of Different Viruses in Patients with Acute Tooth Pain

Detection of Humanherpes Virus-8, Human Cytomegalovirus, and Epstein-Barr Virus in Teeth with Symptomatic Irreversible Pulpitis using the Polymerase Chain Reaction Technique The species and amount of the pathogens, as well as their relationship with each other and the host immunity, can define the variability of clinical symptoms. Therefore, specifying the different microorganisms and their role in endodontic diseases is important to develop novel medicaments. The microbiological samples were obtained from 20 teeth with symptomatic irreversible pulpitis (Group 1).The samples were collected using a sterile paper point from the bleeding pulp chambers of the teeth during the endodontic treatment. The collection protocol of pulp specimens was as follows: After rubber-dam isolation, the crown surface, rubber-dam and clamps were rinsed with 30% H2O2 for 30 s, 2.5% NaOCl for 30 s, and 5% sodium thiosulfate for 30 s. Access cavity was prepared with a sterile bur used in high speed. The same disinfection protocol was used for the dentine just before entering the pulp chamber. When the bleeding pulp was exposed, blood samples were collected with a sterile #30 paper point (DiaDent Europe B.V., Almere, the Netherlands) held in the pulp chamber for 30 s, and then transferred to a sterile Eppendorf tube, and then stored at -20° C. .The sample collection is an easy procedure for an endodontist. All endodontists involved in this study have experience for over three years. The endodontist collected the sample and completed the root canal treatment of volunteer patients in single visit. The procedure was carried out in Marmara University Faculty of Dentistry and all sample collections were completed in one visit.