Soluble fibre metabolites in healthy adults- Part B

This is a double-blinded, randomised, 2-way crossover trial. Participants are required to attend 2 clinic visits. At each clinic visit participants will be randomly allocated to consume one of 2 treatments (2 different soluble fibre formulations, containing 12g of different types of oligosaccharides), plus 2 slices of white bread and 250ml water (room temperature). Study meals will be consumed in random order, with a minimum one-week washout period between clinic visits. Adherence to the intervention will be monitored by direct observation by study personnel. In the two days prior to the scheduled appointments, participants will consume fibre-controlled background diet. This will include consumption of no more than 3 serves of fruits and vegetables combined per day, and avoidance of other high soluble fibre content foods including oats, legumes (kidney, navy, lima, baked beans), wholegrain bread and cereal products. Participants will be provided with a standardised non-fermentable evening meal (lasagna), which they will be asked to consume the night before each of their scheduled appointments. They will also be asked to fast for 12-hours prior to the clinic visit.