Effect of resistant starch on fecal pH, short chain fatty acid concentrations, and gut bacteria in a cohort of normal and stunted children in southern India.

Effect of native and acetylated high amylose maize starch on fecal pH, short chain fatty acid concentrations, and microbiota in a cohort of normal and stunted children in southern India.

Status

Completed

Phases

Unknown

Study type

Interventional

Source

ANZCTR

Registry ID

ACTRN12618000182291

Enrollment

Registered

2018-02-05

Start date

2012-03-05

Completion date

2012-03-21

Last updated

2018-02-12

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

Objective: To compare the effects of two types of resistant starch (RS), high amylose maize starch (HAMS) or acetylated HAMS (HAMSA), on fecal indicators of fermentation in stunted and non-stunted (‘healthy’) children in southern India. Design: Twenty children (10 stunted and 10 healthy) aged 2-to-5 years were fed biscuits containing HAMS (10g/day) for two weeks followed by a 2-week washout and then HAMSA biscuits (10g/day) for 2-weeks. Fecal samples were collected on day 0 and on 12 subsequent occasions at 3-4 day intervals. pH and SCFA were analyzed in all samples, while microbiota were analyzed by Illumina sequencing in 3 children each in stunted and healthy groups.

Interventions

Resistant starch as High Amylose Maize Starch (HAMS) was fed to participants for 2 weeks at 10 grams per day followed by normal diet during the 2 week 'washout' period and then a further 2 weeks of 10 grams per day of Resistant Starch as Acetylated High Amylose Maize Starch (HAMSA). The HAMS and HAMSA were incorporated into biscuits and consumed daily by participants under the supervision of study staff.

Study design

Allocation

Non-randomised trial

Intervention model

Crossover

Primary purpose

Prevention

Masking

Open (masking not used)

Eligibility

Sex/Gender

All

Age

2 Years to 5 Years

Healthy volunteers

Yes

Inclusion criteria

a) Children aged 2-5 years resident in a village in South India (30km from Vellore in the state of Tamil Nadu) b) Weight for Height score (WHZ) between -2 and +2 standard deviations of World Health Organization standards. c) Informed written consent obtained from parent

Exclusion criteria

a) Any chronic illness b) Peripheral edema with WHZ <-2SD or >2 SD of WHO standards

Outcome results