None listed
Conditions
Brief summary
Gut microbiota is highly influenced by environmental factors particularly the diet and its dietary fibre content. Dietary fibre is non-digestible polysaccharide fermented by gut bacteria, subsequently releasing short chain fatty acids, critical for health. Dietary fibre promotes beneficial gut microbiota. However, the potency of different types of fibre to reshape gut microbial population and to boost the release of short chain fatty acids is unknown. While fibre is found in grains and legumes it can be hard to evaluate which type of fibre is present and how much is consumed. The aim of the current study is to test dietary fibre supplements which are affordable and easily quantifiable to determine which type of fibre supplement might be the most beneficial for gut and overall health. 30 women aged between 35-65 years will be recruited. The study will be a randomised open crossover fibre supplementation trial. Each participant will be studied on 3 occasions, such that over the experiment they will receive all 3 fibre supplements in random order. The 3 fibre supplements will include Fibremax (New Image), Vitafiber (Myprotein) and Inulin (Myprotein). Participants will be asked to consume daily the 34g of FibreMax, 15g of Vitafiber or 12g of Inulin supplement per day during the treatment period. Participants will be requested to consume a standardised diet that excludes fibre rich foods (beans, high fibre cereals and bars), dietary supplements, pre and probiotic foods and supplements, antibiotics and anti-inflammatory drugs. Participants will attend an RPA blood collection centre where they will complete 6 x blood collections and the RPA clinic at the Charles Perkins Center on 7 occasions where they will be asked to complete 6 x stool collections and 6 x 4 d food diaries. Participants will first attend a screening interview where they will be asked to complete questionnaires on their medical history, medications, and eating behaviours. Two weeks before the start of the first supplementation period, participants will be asked to consume a standardized diet and will then to undergo 3 x 7 d fibre supplementation periods that will each be separated by 2week washout periods. The blood and stool samples will be used to measure short chain fatty acid levels, microbial diversity in response to dietary fibre supplementation.
Interventions
Healthy subjects, 35-65 years, BMI 18-25kg/m2 will be recruited to determine which type of fibre supplement is more efficient at increasing short chain fatty acids (SCFAs) and bacterial diversity in healthy humans.
Each participant will be studied on 3 x 7 day occasions, such that over the experiment they will receive all 3 fibre supplements in random order. The 3 fibre supplements will include Fibremax (New Image), Vitafiber (Myprotein) and Inulin (Myprotein). Each 7 day fibre supplementation
Healthy subjects, 35-65 years, BMI 18-25kg/m2 will be recruited to determine which type of fibre supplement is more efficient at increasing short chain fatty acids (SCFAs) and bacterial diversity in healthy humans. Each participant will be studied on 3 x 7 day occasions, such that over the experiment they will receive all 3 fibre supplements in random order. The 3 fibre supplements will include Fibremax (New Image), Vitafiber (Myprotein) and Inulin (Myprotein). Each 7 day fibre supplementation period will be separated by a washout period of 2 weeks. Participants will be asked to consume daily the 34g of fibreMax, 15g of Vitafiber or 12g of Inulin supplement per day during the treatment period. Participants will be requested to consume a standardised diet where they will be asked to exclude fibre-rich foods (beans, high-fibre cereals and bars), dietary supplements, pre and probiotic foods and supplements, antibiotics and anti-inflammatory drugs throughout the protocol.. The intervention will be run by a qualified research dietitian with 5 years research experience and qualified research nutritionist with 10 years experience in running dietary interventions. Participants will be asked to keep a 4 day food diary prior to and in the last 4 days of each 7 day fibre supplementation period in addition to keeping a record of the time each fibre supplement was taken and any symptoms. Participants will also be asked to return their fibre supplements for measurement at the end of each 7 day supplementation period.
Sponsors
The University of Sydney
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Prevention
Masking
Open (masking not used)
Eligibility
Sex/Gender
All
Age
35 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
Inclusion criteria: Healthy females, 35-65 years, BMI 18-25kg/m2
Exclusion criteria
Exclusion criteria will include pregnancy or planning pregnancy, breastfeeding, known diabetes, known unstable or untreated blood pressure or cholesterol, cardiovascular disease, chronic inflammatory conditions, medications that may interfere with metabolism, smoking, alcohol consumption above current NHMRC guidelines, allergy or intolerance to foods, irregular eating patterns or eating disorder, following a weight reducing diet, use of antibiotics 4 weeks prior to the start of the study and during the study. Individuals taking anti-inflammatory drugs will also be excluded from the study.
Outcome results
None listed