None listed
Conditions
Brief summary
The aims of this project are to: a. investigate whether the serum composition of post-menopausal women can be altered with 4 weeks of fish oil supplementation and an acute bout of resistance exercise b. Can changes to inflammatory markers in serum alter the activity of muscle cells grown in-vitro Proposal research design: The study is a randomized cross-over design study with a 4 week washout period between groups: Fish Oil group. Participants will be required to ingest 4 fish oil capsules (6g/d containing 2.16g of EPA and 1.42g of DHA) PUFA daily for 4 weeks. This dose has been selected as it is the dosage range recommended by the Australian Heart Foundation for lowering plasma triglyceride concentrations in hypertriglyceridemic subjects and has therefore previously been shown to be physiologically relevant in human subjects. Placebo group. Participants will be required to ingest equivalent amounts of coconut oil (6g/d) for 4 weeks. Coconut oil has been selected as it has been used as a control in other studies involving fish oil. Resistance Exercise. The acute exercise intervention will consist of both upper and lower body exercises including: the leg press, leg extensions, the bench press and dumbbell side lateral raises. The duration of the exercise session, including adequate warm-ups, will be ~60 minutes.
Interventions
All Participants will perform a light session of resistance-based exercise following a thorough warm up. The session of resistance-based exercise consists of machine-based exercises (described below) that provide a supporting surface for balance when performing the exercise. All participants will also undertake pre-testing and measures of body composition one week prior to the trial to ensure the amount of weight lifted during the exercise session can be safely and confidently performed.
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All Participants will perform a light session of resistance-based exercise following a thorough warm up. The session of resistance-based exercise consists of machine-based exercises (described below) that provide a supporting surface for balance when performing the exercise. All participants will also undertake pre-testing and measures of body composition one week prior to the trial to ensure the amount of weight lifted during the exercise session can be safely and confidently performed. This study is a randomised cross over trial, meaning that participants will be randomised into two groups. Either group 1 (fish oil) or group 2 (coconut oil/placebo). After a washout period of 4 weeks, participants will swap groups so that at the end of the trial each participant will have completed a period of fish oil supplementation and a period of coconut oil supplementation. Participants will be randomly allocated to one of the following two experimental: Group 1: Fish Oil supplementation Participants allocated to this group will be required to consume 4 fish oil capsules (6g/d containing 2.16g of EPA and 1.42g of DHA) PUFA daily for 4 weeks. We recommend that participants space their consumption of the fish oil evenly throughout the day and advise participants to take two fish oil capsules in the morning and two capsules in the evening. Side effects: The side effects of fish oil supplementation are rare but may include heartburn, nausea, loose stools and rash. Consumption of fish oil supplements can also lead a fishy after taste that may be unpleasant. The fish oil supplement we have chosen for this study is designed to minimise fishy after taste. If you have a known allergy or hypersensitivity to fish or other fish products (including shellfish), you should be not consuming fish oil supplements. Exercise session The duration of the exercise session, including adequate warm-ups, will be ~60 minutes. The following exercises have been selected as they are low risk and easy for untrained individuals to complete. The following exercises have been selected based on previous established protocols as they are low risk and the most basic exercises for untrained individuals to complete. The following exercises have been used in previous studies thus deemed safe for older adults to perform. Acute Resistance Exercise Session: 1. Leg Press - 3x10 @ RPE 7-8 2. Leg Extensions - 2 x 12 @ RPE 7-8 3. Machine Chest Press - 3 x 10 @ RPE 7-8 4. Dumbbell Side Lateral Raises - 3 x 12 @ RPE 7-8 Resistance exercise will take place at the end of the supplementation periods To monitor adherence to the intervention participants will be asked to return capsules not consumed. Analysis of blood will also determine adherence to the intervention
Sponsors
Bill Tachtsis
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Prevention
Masking
Blinded (masking used) (Subject)
Eligibility
Sex/Gender
All
Age
50 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
i) Female aged 50-65 yo ii) Are currently Post-Menopausal (no periods for 12 and over consecutive months) iii) Have a Body Mass Index (BMI) of 18.5-29 iv) Not exercising more than 3 times per week v) Not currently taking any blood thinning medication e.g. (Warfarin, Aspirin or Dabigatran), anti-inflammatory medication or supplements (anti-allergenic) and not taking fish oil supplements vi) Do not have any known allergies to fish products or coconut oil
Exclusion criteria
Smoker Currently taking taking any blood thinning medication e.g. (Warfarin, Aspirin or Dabigatran), anti-inflammatory medication or supplements (anti-allergenic) and taking fish oil supplements Type 2 diabetes
Outcome results
None listed