None listed
Conditions
Brief summary
Dairy intolerance is often attributed to lactose intolerance but there may be individuals who exhibit an intolerance due to the A1 version of bovine beta-casein protein found in the dairy supply. The peptide sequence variation in the A2 version of beta-casein may alter the digestion but it is still unclear how these variants of the bovine beta-casein in milk are digested and metabolised in humans. This study aims to evaluate digestive, metabolic and immunological responses to milks with or without lactose and A1 beta–casein in self-reported dairy intolerant individuals.
Interventions
750 ml of milk to be consumed on three different occasions separated by 1 week of wash out period between the milk ingestion. Subjects will be fasted overnight prior to each intervention arm. The milk will be consumed in full in the presence of the researchers to confirm compliance, to be ingested within 10 minutes. No additionally food or drink will be consumed during the 3 hour intervention. The milks to be consumed are
(1) Conventional milk (A1+A2 beta-casein)
(2) a2 milk (only A2 beta-case
750 ml of milk to be consumed on three different occasions separated by 1 week of wash out period between the milk ingestion. Subjects will be fasted overnight prior to each intervention arm. The milk will be consumed in full in the presence of the researchers to confirm compliance, to be ingested within 10 minutes. No additionally food or drink will be consumed during the 3 hour intervention. The milks to be consumed are (1) Conventional milk (A1+A2 beta-casein) (2) a2 milk (only A2 beta-casein) and (3) Lactose free milk (A1+A2 beta-casein).
Sponsors
University Of Auckland
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Treatment
Masking
Blinded (masking used) (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
All
Age
20 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
Female aged 20-30years Healthy BMI (18-28kg/m2) No history of gastrointestinal disease or metabolic disease
Exclusion criteria
Allergy to dairy (clinical diagnosis of antibody-mediated milk allergy) Medical history of gastrointestinal disease: celiac, IBS, Crohns and colitis. Fail to produce breath H2 >20ppm after a lactulose challenge. Medical history precluding a healthy state: history of myocardial infarction, angina, stroke, cancer or pre-existing diabetes, Self reported alcohol intake exceeding a moderate intake (>28 units per week).
Outcome results
None listed