None listed
Conditions
Brief summary
Exercise improves health, reduces the incidents of disease and prolongs lifespan. However, the mechanisms through which exercise acts are not clear. A discovery has recently been made in rodents and cells, that mitochondria (the part of the cell that produces energy) can make peptides. Excitingly, these mitochondrial derived-peptides have systemic health promoting effects similar to exercise and may come from skeletal muscle. However, little is known about the factors regulating their levels, particularly in humans. This project aims to improve our understanding of how exercise improves health. If mitochondrial-derived peptides are identified as being endogenously regulated by exercise in humans, they have the potential to become novel targets for preventing and treating various diseases.
Interventions
Participants will attend the laboratory 11 times over 4 weeks. The first visit will be screening and involve a DEXA scan, isometric strength test an incremental exercise test (VO2max test; 10-15 min of stationary cycle exercise) during which respiratory gas will be sampled and heart rate will be monitored. In the second and last (11th) visit participants will undertake a 500 kj (~30-45 min of exercise) performance trial on a stationary exercise bike to assess performance. The remaining visits w
Participants will attend the laboratory 11 times over 4 weeks. The first visit will be screening and involve a DEXA scan, isometric strength test an incremental exercise test (VO2max test; 10-15 min of stationary cycle exercise) during which respiratory gas will be sampled and heart rate will be monitored. In the second and last (11th) visit participants will undertake a 500 kj (~30-45 min of exercise) performance trial on a stationary exercise bike to assess performance. The remaining visits will involve 8-12 repeated 60 s intervals of high-intensity stationary cycling (100% peak power output determined from VO2max test) with 75 s of recovery between each repetition. The number of repeats will start at 8 and be increased dependent on improvements over each session. Each session will be ~30 min and the number of repetitions will increase over the coarse of the two weeks. There will at least 1 day rest between each exercise session. On the 3rd and 10th (second to last) visit we will collect muscle, blood and urine samples before, immediately following and 3 h post exercise. A cannula and/or venepuncher will be used for sampling blood, and a bergstrom biopsy needle for collecting samples from the thigh muscle under local anaesthetic. The interventions will be delivered by trained exercise physiologists.
Sponsors
The University of Auckland
Study design
Allocation
Non-randomised trial
Intervention model
Single group
Primary purpose
Prevention
Masking
Open (masking not used)
Eligibility
Sex/Gender
Male
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
- Male - 18-35 years - Sedentary to moderately active (structured activity of less than 4 hours per week) - Healthy
Exclusion criteria
- Any chronic or advance health conditions or injuries - History of alcohol abuse - Smoking - Taking medications which may affect exercise responses
Outcome results
None listed