None listed
Conditions
Brief summary
We intend to test the hypothesis that consuming gelatin (a food derivative of collagen) and Vitamin C combined with exercise may increase collagen synthesis. To test this hypothesis, eight healthy males will complete a randomized cross-over design protocol where they will consume either a drink containing a isocaloric placebo, 5, or 15 grams of gelatin at a constant level of Vitamin C. Measurements of amino acid appearance in the blood over the first 3 hours will be measured in each condition and a larger blood draw will be taken at 1 hour to determine the effect of the nutritional supplement on engineered ligament collagen and mechanics. The subjects will perform six minutes of rope-skipping to stimulate collagen synthesis. This short bout of exercise will be repeated every six hours for the next three days and will be preceded by one hour with the appropriate drink containing placebo, 5, or 15 grams of gelatin. Over the 3-day training period, blood will be taken for the analysis of the amino-terminal propeptide of collagen 1 (PINP), a byproduct of collagen synthesis.
Interventions
Healthy male subjects will complete a randomized double-blinded cross-over design. Subjects will randomly consume either 5, or 15 grams of gelatin or an isocaloric placebo control. Subjects will consume each treatment orally mixing the treatment from an opaque sachet in a Vitamin C enriched (48mg) flavoured beverage (400ml). Subjects will be instructed to mix the beverage in a opaque bottle and consume as quickly as possible. Each treatment will be consumed 1 hour prior to the completion of 6 m
Healthy male subjects will complete a randomized double-blinded cross-over design. Subjects will randomly consume either 5, or 15 grams of gelatin or an isocaloric placebo control. Subjects will consume each treatment orally mixing the treatment from an opaque sachet in a Vitamin C enriched (48mg) flavoured beverage (400ml). Subjects will be instructed to mix the beverage in a opaque bottle and consume as quickly as possible. Each treatment will be consumed 1 hour prior to the completion of 6 minutes of self paced skipping. Subjects will complete each treatment of the investigation and have a 4 day wash out period between crossing over to an alternate treatment. In Lab acute ingestion and exercise phase At baseline and 1 hour after the ingestion of the initial treatment (single dose prior to skipping) a large blood sample (50ml) will be taken, Sterile serum from these blood samples will be used for the treatment of engineered ligaments. Measurements of collagen content and mechanical properties of the engineered ligaments will be undertaken. Blood samples will be taken at 0, 30min, 1, 1.5, 2 and 3hours post ingestion of each treatment for the analysis of serum amino acids concentrations. Four hours post exercise a final blood sample will be collected for post exercise serum amino-terminal propeptide of collagen I (PINP) analysis before the subject will be allowed to leave the lab. Supplementation and exercise phase Immediately following the above acute ingestion investigation phase subject will be required to repeat the intervention (treatment ingestion 1 hour prior to 6 minutes of self paced rope-skipping) 3 times a day with at least 6 hours between exercise bouts for 3 days. Subjects will return to the lab at 24, 48, and 72 hours post initial exercise and ingestion of treatment for determination of serum PINP concentrations. Subjects adherence to treatment ingestion and exercise will be completed at daily blood collection time points through verbal feedback and recorded by the researcher. As above subjects will have a 4 day wash out period before crossing over to on of the other randomly assigned treatments.
Sponsors
Australian Institute of Sport
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Treatment
Masking
Blinded (masking used) (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
Male
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
Active individuals exercising 2-3x per week.
Exclusion criteria
History of recurrent connective tissue, tendon or ligament injury or current injury which prevents the exercise task being completed. Known allergy to animal gelatin.
Outcome results
None listed