None listed
Conditions
Brief summary
The project aims to determine the effect that physical forms of foods have on the kinetics/mechanics of absorption of nutrients from processed foods in the small intestine.
Interventions
The uptake of 10 g lactulose and 5 g mannitol incorporated into (a) 50 g of uncooked extruded pasta pellets (mean diameter: 3.18 millmetre ) made of maize flour (b) 50 g of uncooked hydrated (1:2 w/w in water) powdered pasta pellets and (c) a control consisting of 50 ml of the two probe sugars dissolved in water. All of these interventions were mixed with 150 g of 'fruit sauce' containing 0.1 % Guar gum (Grindsted (Registered Trademark), DaniscoPvt Ltd, Australia), 0.3 % Xanthan (80 Mesh, ISXA
The uptake of 10 g lactulose and 5 g mannitol incorporated into (a) 50 g of uncooked extruded pasta pellets (mean diameter: 3.18 millmetre ) made of maize flour (b) 50 g of uncooked hydrated (1:2 w/w in water) powdered pasta pellets and (c) a control consisting of 50 ml of the two probe sugars dissolved in water. All of these interventions were mixed with 150 g of 'fruit sauce' containing 0.1 % Guar gum (Grindsted (Registered Trademark), DaniscoPvt Ltd, Australia), 0.3 % Xanthan (80 Mesh, ISXANT8.1, IngredientStop) and 0.02 % vanilla flavouring (Batch 181110, Zymus) and a standard volume of water. Lactulose and mannitol was determined by measuring their excretion in urine by high performace liquid chromatography (HPLC). These foods each were fed to 22 healthy female participants (screened by questionnaire) in random sequence at weekly intervals. The subjects were asked to empty their bladder every half-hour over a six hour period. Venous blood was collected every half-hour over four hours and thereafter at five and six hours for plasma blood glucose and plasma insulin determination. Comparisons of the temporal pattern of absorption of each of the two probe sugars sugar and the blood glucose values for six hours after consumption of each food with that of the control will provide an indication of the effect of processing on the rates of absorption of nutrients from the gut lumen.
Sponsors
Massey University
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Treatment
Masking
Blinded (masking used) (Subject)
Eligibility
Sex/Gender
All
Age
20 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
Female volunteers between the age of 20 and 40 years were screened by questionnaire for general medical health, gut health, ongoing consumption of medication, dietary supplements, dietary peculiarities and smoking. Inclusion criteria is as follows: (1) Participants were non-smokers with no history of gastrointestinal diseases including recent gut infection evidenced by a recent history of abdominal pain, nausea, vomiting, diarrhea, passage of blood and mucus in stools. (2) Participants were not taking any ongoing prescription or OTC medication or multivitamins except for contraceptive pills. (3) The number of Standard alcohol drinks consumed per week were calculated from results of alcohol consumption in the questionnaire to ensure that participants did not exceed a moderate alcohol intake. (4) Participants did not have high levels of endogenously produced mannitol or lactulose in the urine.
Exclusion criteria
Participants were screened by a Health Questionnaire (attached) to determine participation in the study. Volunteers who were excluded are: Those that had high levels of endogenously produced mannitol or lactulose in the urine, smokers, exceeded the intake of one standard alcohol drink per day, had a history of gastrointestinal diseases including recent gut infection evidenced by a recent history of abdominal pain, nausea, vomiting, diarrhea, passage of blood and mucus in stools, had a history of or current urinary tract infections, vaginal conditions that cause discharge, took ongoing prescription medication, antibiotics, OTC medication or multivitamins, took prebiotic and probiotic supplements such as lactulose.
Outcome results
None listed