None listed
Conditions
Brief summary
Dairy protein is know to stimulate muscle protein synthesis (MPS) and resistance exercise is know to act synergistic to further increase MPS. It is not known if a small dose of dairy protein (9 g) can increase MPS. It is hypothesized that 9 g of dairy protein will increase MPS at rest and that MPS with be further increased when 9 g of dairy protein is combined with resistance exercise.
Interventions
Consumption of a formulated milk beverage containing 9 grams of dairy protein, 13g of carbohydrate and 3.2 g of lipid dissolved in 200 ml of water. Energy =504kJ
At baseline all participants will undergo DXA and pqCT scans to estimate body composition on muscle size as well as isometric strength measurements.. Participants will undergo a primed constant infusion of 13C6 phenylalanine for three hours in the rested fasted state before a muscle biopsy will be obtained from the vastus lateralis to
Consumption of a formulated milk beverage containing 9 grams of dairy protein, 13g of carbohydrate and 3.2 g of lipid dissolved in 200 ml of water. Energy =504kJ At baseline all participants will undergo DXA and pqCT scans to estimate body composition on muscle size as well as isometric strength measurements.. Participants will undergo a primed constant infusion of 13C6 phenylalanine for three hours in the rested fasted state before a muscle biopsy will be obtained from the vastus lateralis to calculate muscle protein synthesis (MPS). They will then preform 3 sets of unilateral knee extension and leg press for a randomly selected leg. They will then consume either the treatment or placebo beverage. Additional biopsies will be obtained from each leg at 90 and 210 min after beverage consumption while the isotope infusion is maintained. MPS will be calculated form 0-90 and 9-210 min after beverage consumption. .
Sponsors
David Cameron-Smith
Study design
Allocation
Randomised controlled trial
Intervention model
Parallel
Primary purpose
Prevention
Masking
Blinded (masking used) (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
Male
Age
35 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
BMI (18-30kg/m2) Sedentary to moderately active
Exclusion criteria
1.Diabetes or symptoms of metabolic syndrome or current pharmaceutical therapy 2. Other medical conditions impacting on digestion, including diseases of the gastrointestinal tract, allergy to dairy protein and history (or medical management) of mental illness 4. Smoking
Outcome results
None listed