None listed
Conditions
Brief summary
The purpose of the study is to determine the permeability of the gut in health and in Crohn's disease and the concentration of bacterial endotoxin in the systemic circulation in order to assess whether a leaky gut is accompanied with greater absorption of bacterial products e.g. endotoxin.
Interventions
A venous blood sample from each participant will be drawn on arrival at our research facilities. Each participant will drink 100 ml of a lactulose- mannitol solution containing 10 g of lactulose and 5 g of mannitol immediately followed by 300 ml of water. Participants will be ask to to empty their bladder on arrival and at 1.5 and 3 hours after the consumption of the lactulose-mannitol solution.
Sponsors
Massey University
Eligibility
Sex/Gender
All
Age
20 Years to 60 Years
Healthy volunteers
No
Inclusion criteria
for Crohn's disease group - Diagnosis of Crohn’s disease at least 6 months prior to the study - either being in remission or having mild and moderate disease activity as assessed by the Harvey-Bradshaw Index (HBI) (remission: HBI <5, moderate disease activity: HBI 5-8, mild disease activity: HBI 8-16)
Exclusion criteria
- Have any dietary excesses or particularities e.g. food allergies - Drink more than one alcoholic standard drink per day (or more than seven alcoholic standard drinks per week) - Are pregnant or lactating - Are taking vitamin/ mineral supplements - Have a history of diabetes, renal (kidney) disorders or cancer - Have an aversion to venepuncture, difficult veins, a history of bleeding disorders or on anticoagulant therapy - recent consumption of foods containing lactulose or mannitol additional exclusion criteria for healthy volunteers: - Have a medical or family history of Crohn’s disease, ulcerative colitis or irritable bowel disease - Have a recent history of abdominal pain, nausea, vomiting, diarrhoea, passage of blood and mucus in stool
Outcome results
None listed