None listed
Conditions
Brief summary
In an 8 week randomised controlled trial, patients with type 2 DM (n=30) will be randomised into one of two treatment groups to receive either exercise and 30 mg/d elemental zinc (‘Zn + exercise group’) or exercise and placebo (‘Exercise group’). Supplementation will occur over the 8 week intervention, with exercise training conducted in the last week of the study period. All participants will undergo supervised daily exercise training for 60 min/day at 60% of maximal fitness capacity for 7 consecutive days. During the study, participants will be asked to provide three fasting blood samples in total (baseline, week 6 and week 8). Blood samples will be analysed for glucose, insulin, lipids and zinc. Peripheral blood mononuclear cells (PBMC) will be isolated for the assessment of cellular zinc status which includes the expression of zinc transporter, metallothionein, and mediators of insulin sensitivity will be measured. Insulin sensitivity will be measured by hyperinsulinemic, euglycaemic clamps on three occasions (baseline, week 6 and week 8) during the intervention period. A skeletal muscle biopsy may be obtained during the clamp, with participant's additional consent.
Interventions
30 mg/d elemental zinc supplementation for 8 weeks delivered orally in capsule form. Compliance will be checked by the count of capsule return
Aerobic exercise training supervised by an exercise scientist for 60 min/day at 60% of maximal fitness capacity on treadmill for 7 consecutive days in the last week of study.
Sponsors
University of Otago
Study design
Allocation
Randomised controlled trial
Intervention model
Parallel
Primary purpose
Treatment
Eligibility
Sex/Gender
All
Age
18 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
Patients diagnosed with type 2 DM who are physically inactive (n=30)
Exclusion criteria
Exclusion criteria include: habitual smokers, women who are pregnant or breastfeeding, those with diagnosis of any other major illness, the use of vitamins or mineral supplements in the last 6 weeks and medical contraindication to maximal exercise testing or exercise training.
Outcome results
None listed