None listed
Conditions
Brief summary
Cultivation of embryos in the new incubator using 5% O2, unlike the previous 20% O2, opens the question whether this increases biological potential of embryos for implantation or not and which methods for determination of this potential are more accurate and reliable. Given that the existing data on the benefits of cultivation with 5% O2 are contradictory considering the age of the patient, it is necessary to carry out research on a larger number of samples of all ages. Current methodology for determining the biological potential of embryos -morphology assessment should be supplemented by an objective method that is not subject to the subjective judgment of biologists –embryologist. Such a potential method is determination of the metabolic profile of amino acids of embryos by analyzing the spent media in which they are cultivated. It is necessary to determine the sensitivity and applicability of such methods. Given the fact that we work with human material it is necessary that a method is absolutely non-invasive.
Interventions
Randomized cultivation of human embryos with different levels of oxygen (5% versus 20%) from the day of fertilization (day 0) till the day of embryo transfer (day 5). On the day 3, the best embryos (based on morphokinetics) will be cultivated separately till the day 5. On the day 5, blastocyst media of the transffered embryo will be analyzed by LC-MS/MS ( liquid chromatography-mass spectrometry) to identify specific metabolic amino acid profile of the transffered embryos.
Sponsors
NINA GELO
Study design
Allocation
Randomised controlled trial
Intervention model
Parallel
Primary purpose
Treatment
Masking
Open (masking not used)
Eligibility
Sex/Gender
All
Age
22 Years to 42 Years
Healthy volunteers
No
Inclusion criteria
Infertile patients in stimulated cycles
Exclusion criteria
Severe male infertility (azoospermia, cryptozoospermia)
Outcome results
None listed