Does an innovative method of sequencing, known as next generation sequencing (NGS), applied for normal embryo selection in infertile patients improve pregnancy rate in frozen transfer?

In patients with fertility problems, does next generation sequencing-based preimplantation genetic diagnosis followed by frozen blastocyst embryo transfer, compared to group without preimplantation genetic diagnosis, improve pregnancy rate?

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ANZCTR

Registry ID

ACTRN12614001037695

Enrollment

114

Registered

2014-09-25

Start date

2013-08-01

Completion date

2014-01-01

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

Our objective was to determine pregnancy rate after comprehensive chromosome screening (CCS) using PGD NGS in frozen embryo transfer cycles after blastocyst’s trophoectoderm biopsy. 40 patients who decided to undergo PGD on 24 chromosomes on blastocystes with vitrification and frozen embryo transfer were included in the study as an investigated group. As a control group we selected 74 cases which were comparable in age, anty-mullerian hormone level (AMH), antral follicle count (AFC) and causes of infertility. Only first IVF cycles were taken under consideration.

Interventions

Preimplantation genetic diagnosis using next generation sequencing. Involves patient's biopsied embryos genetic analysis. Choice of the embryos for transfer was based on the PGD NGS results. Duration: 12 hours of analysis Frequency: Once in this investigation

Study design

Allocation

Non-randomised trial

Intervention model

Parallel

Primary purpose

Diagnosis

Masking

Open (masking not used)

Eligibility

Sex/Gender

All

Age

33 Years to 39 Years

Healthy volunteers

Inclusion criteria

1. Patients undergoing intracytoplasmic sperm injection (ICSI) in Invicta Fertility and Reproductive Centre s. z o.o. in time frame from 08.2013 to 01.2014 2. At least two previous implantation failures

Exclusion criteria

1. Patients with endometriosis or adenomiosis 2. Patients with problems related to endometrium thickness

Outcome results