Does an innovative method of sequencing, known as next generation sequencing (NGS), applied for normal embryo selection in infertile patients improve pregnancy rate in fresh transfer?

In patients with fertility problems, does preimplantation genetic diagnosis, compared to group without preimplantation genetic diagnosis, improve pregnancy rate?’

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ANZCTR

Registry ID

ACTRN12614001035617

Enrollment

Registered

2014-09-25

Start date

2013-08-01

Completion date

2014-07-01

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

Objective: To determine the usefulness of semiconductor-based next generation sequencing (NGS) for cleavage-stage preimplantation genetic diagnosis (PGD) of aneuploidy. Setting: A private centers for reproductive medicine. Patient(s): Patients underwent day-3 embryo biopsy with PGD and fresh cycle transfer. Additionaly, 53 patients, matched according to age, anti-mullerian hormone (AMH) levels, antral follicles count (AFC) and infertility duration were selected to serve as controls. Intervention(s): Choice of the embryos for transfer was based on the PGD NGS results. Main Outcome Measure(s): Clinical pregnancy rate (PR) per embryo transfer was our primary outcome. Secondary outcomes were the implantation rate, and miscarriage rate.

Interventions

Involves patient's biopsied embryos genetic analysis. Choice of the embryos for transfer was based on the preimplantation genetic diagnosis using next generation sequencing (PGD NGS) results. Duration: 12 hours of analysis Frequency: Once in this investigation

Study design

Allocation

Non-randomised trial

Intervention model

Parallel

Primary purpose

Diagnosis

Masking

Open (masking not used)

Eligibility

Sex/Gender

All

Age

30 Years to 38 Years

Healthy volunteers

Inclusion criteria

1. Patients undergoing intracytoplasmic sperm injection (ICSI) in Invicta Fertility and Reproductive Centre s. z o.o. in time frame from 08.2013 to 01.2014 2. At least two previous implantation failures

Exclusion criteria

1. Patients with endometriosis or adenomiosis 2. Patients with problems related to endometrium thickness

Outcome results