None listed
Conditions
Brief summary
study of the effect of the presence of gene mutation on development of preeclampsia and determination of its severity
Interventions
A prospective case control study
Will include 120 preeclamptic patients will be subdivided into mild and severe groups according to blood pressure and proteinuria .2 ml venous blood samples were taken once from the patient under a septic technique on Vaccutainer Ethylenediaminetetraacetic acid (EDTA) tubes . Deoxyribonucleic acid (DNA) Extraction and Purification using a DNA salting-out technique.Detection of C677T MTHFR gene polymorphis ,G1691A Factor V Lieden , G20210A Prothrombin genes
A prospective case control study Will include 120 preeclamptic patients will be subdivided into mild and severe groups according to blood pressure and proteinuria .2 ml venous blood samples were taken once from the patient under a septic technique on Vaccutainer Ethylenediaminetetraacetic acid (EDTA) tubes . Deoxyribonucleic acid (DNA) Extraction and Purification using a DNA salting-out technique.Detection of C677T MTHFR gene polymorphis ,G1691A Factor V Lieden , G20210A Prothrombin genes and insertion /deletion(I/D) of ACE polymorphisms were done.
Sponsors
alexandria university , faculty of medicine
Eligibility
Sex/Gender
All
Age
20 Years to 38 Years
Healthy volunteers
No
Inclusion criteria
normotensive pregnant female in third trimester mild preeclampsia in third trimester severe preeclampsia in third trimester
Exclusion criteria
other hypertensive diseases with pregnancy
Outcome results
None listed