The effect of different media for sperm handling and injection at intracytoplasmic sperm injection (ICSI) on subsequent embryo cleavage: a comparison of the use of polyvinylpyrrolidone (PVP) and hyaluronan.

Morphokinetic comparisons of embryos derived from intracytoplasmic sperm injection (ICSI) procedures involving polyvinylpyrrolidone (PVP) or hyaluronan as sperm handling reagent - a randomized sibling oocyte study

Status

Completed

Phases

Unknown

Study type

Interventional

Source

ANZCTR

Registry ID

ACTRN12613000957796

Enrollment

Registered

2013-08-28

Start date

2014-07-14

Completion date

2015-03-11

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

Polyvinylpyrrolidone (PVP) has been routinely used in the intracytoplasmic sperm injection procedures to facilitate sperm handling for years although it's not degradable by the egg. Hyaluronan is a new alternative to PVP with all the features that PVP has and it's degradable by the egg. The proposed study aims at comparing the embryonic effect between these 2 reagents by using time-lapse analysis.

Interventions

Sibling oocytes collected from each participating patient will be randomly allocated into the polyvinylpyrrolidone (PVP) (control) and hyaluronan (HA) (treatment) groups. Oocytes will undergo intracytoplasmic sperm injection (ICSI) with different handling medium in each group accordingly. Subsequent embryo culture condition is identical for both groups.

Study design

Allocation

Non-randomised trial

Primary purpose

Treatment

Eligibility

Sex/Gender

All

Age

18 Years to 45 Years

Healthy volunteers

Inclusion criteria

All patients having ICSI treatment criteria at Fertility North. Indications for ICSI include low sperm count, low sperm motility, poor sperm morphology or poor fertilisation in previous conventional insemination.

Exclusion criteria

Patients requiring sperm selection using PICSI dishes, eg male partner with increased incidence of sperm DNA fragmentation.

Outcome results