None listed
Conditions
Brief summary
PICSI dish and Spermslow (liquid hyaluronan) are both hyaluronan binding-based sperm selection methods. Publications have shown benefits of sperm selection by either method before intracytoplasmic sperm injection. However few study has compared the efficacy between these two methods. The proposed study will compare sibling embryos resulted from these two methods by using time-lapse analysis.
Interventions
PICSI dish and Spermslow media are both designed for sperm selection prior to intracytoplasmic sperm injection (ICSI). They are both based on the hyaluronan-sperm binding assay but with different protocols. The former is coated with solid hyaluronan while the latter is the liquid status hyaluronan. Mature sperm tend to lose its progrssive motility when meet hyaluronan while immature ones don't. Sibling oocytes collected from each participating patient will be randomly allocated into the PICSI (
PICSI dish and Spermslow media are both designed for sperm selection prior to intracytoplasmic sperm injection (ICSI). They are both based on the hyaluronan-sperm binding assay but with different protocols. The former is coated with solid hyaluronan while the latter is the liquid status hyaluronan. Mature sperm tend to lose its progrssive motility when meet hyaluronan while immature ones don't. Sibling oocytes collected from each participating patient will be randomly allocated into the PICSI (control) and Spermslow (treatment) groups. Prepared sperm sample will be split and then undergo either selection methods (control or treatment group) prior to ICSI. Selected sperm from each group will be used for ICSI with sibling oocytes. After injection, comparisons will be made on ferilisation rate, time post ICSI insemination to reach syngamy, 2,3,4,5,6,7,8-cell stages (t2,t3,t4,t5,t6,t7,t8)(hours), and implantation results after transfer of embryos. Only one-off intervention is involved at the sperm/egg level, none at the patient level.
Sponsors
Fertility North
Study design
Allocation
Non-randomised trial
Primary purpose
Treatment
Eligibility
Sex/Gender
All
Age
18 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
All patients who meet the PICSI treatment criteria according to Fertility North protocols, including high sperm DNA fragmentation or poor result in previous ICSI treatment.
Exclusion criteria
Poor semen quality (motile sperm count less than 1 million) or poor ovarian response (less than 2 oocytes collected)
Outcome results
None listed