None listed
Conditions
Brief summary
Both Swim-up and Density gradient centrifugation are routine sperm preparation methods in IVF laboratory. However the content of seminal fluid might mix with separated sperm in the Swim-up method while centrifugation is involved in the other method which are both considered harmful to fertilisation and embryo development. The proposed study will use time-lapse technology to reveal the differeces in the subsequent embryo development.
Interventions
Swim-up (SU) and Density Gradient centrifugation (DGC) are both routinely used sperm preparation methods during IVF procedure. SU involves layering semen sample and culture media, and active swimming-up of sperm into the media phase so that sperm with good motility can be yielded. However there is a risk that seminal components migrate into the media which is believed to impact fertilisation and subsequent embryo growth. DGC involves centrifugations to force the motile sperm to go through densit
Swim-up (SU) and Density Gradient centrifugation (DGC) are both routinely used sperm preparation methods during IVF procedure. SU involves layering semen sample and culture media, and active swimming-up of sperm into the media phase so that sperm with good motility can be yielded. However there is a risk that seminal components migrate into the media which is believed to impact fertilisation and subsequent embryo growth. DGC involves centrifugations to force the motile sperm to go through density gradient media and then get separated. But centrifugations are proven to be harmful to sperm integrity and in turn affect fertilisation and subsequent embryo development. DGC is currently used as standard method for sperm preparation at Fertility North and will be used as control group. SU will be the treatment group. Sibling oocytes collected from each participating patient will be randomly allocated into DGC (control) and SU (treatment) groups. Semen sample provided by the male partner will be split and go through different preparation methods separately. Oocytes in each group will be inseminated with sperm prepared by according method. After insemination, comparisons will be made on ferilisation rate, time post insemination to reach syngamy, 2,3,4,5,6,7,8-cell stages (t2,t3,t4,t5,t6,t7,t8)(hours), and implantation results after transfer of embryos. Only one-off intervention is involved at the sperm/egg level, none at the patient level.
Sponsors
Fertility North
Study design
Allocation
Non-randomised trial
Primary purpose
Treatment
Eligibility
Sex/Gender
All
Age
18 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
All patients who meet the IVF treatment criteria according to Fertility North protocols, including tubal factor, PCOS, low AMH or multiple failures in previous IUI treatment.
Exclusion criteria
Semen quality of partner not within normal range
Outcome results
None listed