The effect of remote ischaemic preconditioning on the late immune response and nervous system

The effect of remote ischaemic preconditioning on the second window immune response and parasympathetic nervous system in healthy volunteers

Status

Not yet recruiting

Phases

Unknown

Study type

Interventional

Source

ANZCTR

Registry ID

ACTRN12613000535774

Enrollment

Registered

2013-05-14

Start date

2013-07-01

Completion date

Unknown

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

Remote ischaemic preconditioning (RIPC) is the phenomenon where brief periods of ischaemia (reduced oxygen delivery) in one organ can have a protective effect against subsequent ischaemia in other organs. There are two periods of protection provided by RIPC. The first occurs within minutes and lasts between two and four hours, whereas the second presents 24 hours after the conditioning and persists for up to 72 hours. The exact mechanisms through which RIPC exerts these protective periods are unclear; however, there is evidence to support the involvement of both immune and nervous pathways. This study will investigate the effect of RIPC on the immune response during the late phase of protection, and on the immediate parasympathetic nervous response. This will be achieved by recruiting 15 healthy male volunteers and collecting blood samples and electrocardiogram traces of the electrical activity of the heart before, and 24 hours after, RIPC. The blood samples will be used to measure the effect of RIPC on inflammatory biomarker levels and the function of circulating white blood cells. The ECGs will be used to determine if the RIPC stimulates the parasympathetic nervous system.

Interventions

Remote ischaemic preconditioning will be performed at the start of the study. A blood pressure cuff will be placed on the non-dominant upper arm and inflated to 200 mmHg for five minutes, then deflated for five minutes. This cycle will be performed three times immediately one after the other, taking a total of thirty minutes. Follow-up will take place up to 24 hours post-treatment.

Study design

Allocation

Non-randomised trial

Intervention model

Single group

Primary purpose

Treatment

Masking

Open (masking not used)

Eligibility

Sex/Gender

Male

Age

18 Years to 30 Years

Healthy volunteers

Yes

Inclusion criteria

Healthy volunteers

Exclusion criteria

Peripheral vascular disease Smoking Regular use of medication Acute illness (within 1 week of the study visits) Any cardiac rhythm abnormality Any condition affecting the autonomic nervous system (eg diabetes, autonomic neuropathy)

Outcome results