Microscopic comparison of samples of small intestine between critically ill patients and non-critically ill patients

Retrospective cohort study to determine if ultrastructural changes are present in duodenal biopsies of critically ill patients compared to ambulant volunteers

Status

Not yet recruiting

Phases

Unknown

Study type

Observational

Source

ANZCTR

Registry ID

ACTRN12613000508774

Enrollment

Registered

2013-05-08

Start date

2013-06-01

Completion date

Unknown

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

We hypothesize that ICU patients have changes in the structure of their small intestinal cells that predisposes them to malabsorption of nutrients. To investigate this, we are comparing biopsies from 15 ICU patients and comparing them to biopsies from 15 healthy patients. We will analyse the samples with an electron microscope.

Interventions

Comparison of duodenal ultrastructure between ambulant volunteers and critically ill patients. The duodenal samples from critically ill patients have already been collected as part of a previous study (Royal Adelaide Hospital Research Ethics Committee protocol number 051211). 6 duodenal samples were taken per patient for 15 patients, over the period of 2011-2012. Therefore, 6 duodenal samples will be taken from each ambulant volunteer, and the total length of the endoscopy will not be signific

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

Ambulant volunteers who are planned to undergo an endoscopy for clinical reasons

Exclusion criteria

Inability to provide informed consent Known small intestinal pathology including coeliac disease, inflammatory bowel disease or cystic fibrosis Suspicion of small intestinal pathology including coeliac disease Active gastrointestinal bleeding Ingestion, within the previous 7 days, of drugs known to alter platelet aggregation or thrombus formation, including: i. Aspirin > 150mg/day; ii. Any other non-steroidal anti-inflammatory drug; iii. Thienopyridines (clopidogrel) Patients on antibiotics known to effect gastrointestinal function Patients whose religious belief prevents administration of blood product e.g. Jehovah’s Witness Chronic alcohol abuse (> 5 standard drinks per day) Previous bariatric or small intestinal surgery other than appendicectomy Pregnancy or breast-feeding

Outcome results