The effect of anterior cruciate ligament injury on skeletal muscle function, contractile proteins and inflammation

Skeletal muscle biopsy to evaluate Na+, K+, and ATPase content in young ACL injured participants

Status

Completed

Phases

Unknown

Study type

Interventional

Source

ANZCTR

Registry ID

ACTRN12613000157774

Enrollment

Registered

2013-02-08

Start date

2013-03-04

Completion date

2014-12-01

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

Damage to the Anterior cruciate ligament (ACL), a ligament vital for the stability of the knee joint, affects the ability to perform daily activities and physical exercise. The reduced physical activity caused by knee injury can cause changes in the muscle of the leg, causing decreased muscle strength, size and endurance. This study proposes to test the effect of ACL injury (knee injury) on muscle on a microscopic or molecular level, and investigate how such molecular changes in the muscle can affect knee function. A second aim of the study is to investigate the role of inflammation in the knee joint and how it affects leg strength and physical activity levels. This will be achieved by measuring strength, balance, physical activity levels, and muscle samples in patients undergoing ACL reconstructive surgery compared to a matched control group. It is hypothesized that after acute ACL trauma, functional changes in quality of life and muscle function of the injured limb will be associated with adverse changes in knee extensor muscle contractility on a biochemical level.

Interventions

No drug intervention involved. Muscle sample taken via muscle biopsy with a Bergstrom needle from the vastus lateralis of both injured and uninjured legs of patients undergoing ACL reconstructive surgery. The biopsy procedure takes less than 5 minutes on each leg.

Study design

Allocation

Non-randomised trial

Intervention model

Other

Primary purpose

Diagnosis

Masking

Open (masking not used)

Eligibility

Sex/Gender

All

Age

18 Years to 35 Years

Healthy volunteers

Inclusion criteria

ACL group: 1. Scheduled for ACL reconstructive surgery. 2. Between 18-35 years of age 3. BMI <30 Control Group: 1. Free from lower limb injury 2. Between 18-35 years of age 3. BMI <30

Exclusion criteria

ACL group: Not between 18-35 years of age. Any metabolic or cardiovascular diseases which impact on physical activity prior to ACL injury. Control group Not between 18-35 years of age Any metabolic or cardiovascular diseases which impact on physical activity levels Recent lower limb joint injury

Outcome results