None listed
Conditions
Brief summary
The aim of our study is to identify each of the three categories of CPPS II, IIIA and IIIB patients according to NIDDK (NIH) classification, to investigate their sperm DFI and to correlate it with potential abnormalities in their sperm, with the DFI of general and infertile men population, thus to provide useful informations for potential association of CPPS and male infertlity.
Interventions
Chronic prostatitis-Chronic pelvic pain syndrome (CPPS)patients in II, IIIa and IIIb categories according to NIDDK (NIH) classification, investigated for sperm abnormalities and correlation of these abnormalities with DNA Fragmentation Index (DFI) determinated by TUNEL technique
TUNEL is a common method for detecting DNA fragmentation that results from apoptotic signaling cascades. The assay relies on the presence of nicks in the DNA which can be identified by terminal deoxynucleotidyl transfera
Chronic prostatitis-Chronic pelvic pain syndrome (CPPS)patients in II, IIIa and IIIb categories according to NIDDK (NIH) classification, investigated for sperm abnormalities and correlation of these abnormalities with DNA Fragmentation Index (DFI) determinated by TUNEL technique TUNEL is a common method for detecting DNA fragmentation that results from apoptotic signaling cascades. The assay relies on the presence of nicks in the DNA which can be identified by terminal deoxynucleotidyl transferase or TdT, an enzyme that will catalyze the addition of dUTPs that are secondarily labeled with a marker. It may also label cells that have suffered severe DNA damage. This examination is going to be performed the 3-5 days after the first clinical examination Every patient is going to be observed for 7-10 days, time necessary to have the final results of the sperm culture, urine analysis and culture, spermogram, and estimation of DNA fragmantation Index.
Sponsors
General Hospital of Thessaloniki “G. Gennimatas”
Eligibility
Sex/Gender
Male
Age
18 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
- History of chronic pelvic pain for three or more months - Rating Questionnaire «NIH Chronic Prostatitis Symptom Index», over 3 (this Questionnaire is composed by 9 questions relative with the modality of pain, the sense of urine and the quality of life) - Physical and mental condition of the patient such that they can cooperate with the physician-researcher.
Exclusion criteria
- Suffering from an acute infection of the genitourinary - Undergoing Chemotherapy - Suffering from varicocele - Have been treated for in less than three months for varicocele, torsion or testicular injury - History of cryptorchidism - Hisrory of testicular cancer - Subjected to some systemic medication the last 3 months - Smoking more than 10 cigarettes a day - Clinical signs of epididymitis
Outcome results
None listed