None listed
Conditions
Brief summary
The aim of this project is to understand whether specific FTO risk gene variants influence the skeletal muscle’s ability to utilise carbohydrates and fats at a genetic and tissue level following a 12 week high intensity intermittent exercise training intervention. Ten (10) healthy males with the FTO rs9939609 risk allele (A) and 10 healthy males with two FTO rs9939609 wild type alleles (TT) between the ages 20 - 50 will be recruited to participate in this study. Following a buccal swab and venous blood sampling to obtain a genetic and metabolic signature profile, participants will perform a low (40% VO2max-LO) and high-intensity (80% VO2max-HI) exercise bout for approximately 70 and 38 minutes, respectively. This second test will occur a minimum of 2 days following the commencement of the first exercise test (maximum of up to 1 week). Venous blood will be sampled prior to, immediately after, 15 minutes, and 3 hours following completion of the exercise. Urine samples will be collected prior to and 3 hours following the completion of the exercise. A 12 week exercise training intervention will then be employed. 36 training sessions will be conducted in total over the 12 week period. This intervention will involve participants cycling at 150% VO2max for 20 seconds with 40 seconds passive recovery per minute, and the frequency and duration of sessions will be incremented in 4 x 3 week blocks. Following this intervention the LO and HI exercise tests will be repeated (following the same procedures as indicated above).This project will provide important information on whether low or high intensity exercise is more effective to stimulating glucose and fat metabolism in skeletal muscle, and whether high intensity intermittent exercise training can influence the metabolic profile of those with FTO obesity risk variants. This project may also provide genetically susceptible individuals with an insight in to risk-reducing behaviours that can be effective in prevention of obesity.
Interventions
Participants will be asked to submit to a 12 week exercise training intervention. Thirty six (36) training sessions will be conducted in total over the 12 week period. This intervention will involve participants cycling at 150% VO2max for 20 seconds with 40 seconds passive recovery per minute during each session. The frequency and duration of sessions will be incremented in 4 x 3 week blocks (weeks 1-3, 2 x 20 minutes per week; weeks 4-6, 3 x 20 minutes per week; weeks 7-9, 3 x 30 minutes per we
Participants will be asked to submit to a 12 week exercise training intervention. Thirty six (36) training sessions will be conducted in total over the 12 week period. This intervention will involve participants cycling at 150% VO2max for 20 seconds with 40 seconds passive recovery per minute during each session. The frequency and duration of sessions will be incremented in 4 x 3 week blocks (weeks 1-3, 2 x 20 minutes per week; weeks 4-6, 3 x 20 minutes per week; weeks 7-9, 3 x 30 minutes per week; weeks 10-12, 4 x 30 minutes per week). Prior to and following the 12 week exercise training intervention participants will be asked to perform exercise tests during two testing sessions (total of four testing sessions). One exercise test will involve a single bout of cycle ergometer exercise at 80% (HI) VO2max for 38 minutes which expends ~400 kcal. This is a crossover study with the order of the exercise tests being randomised. The second test will occur a minimum of 2 days following the commencement of the first exercise test (maximum of up to 1 week). The second exercise test will involve a single bout of cycle ergometer exercise at 40% (LO) VO2max for 70 minutes which expends ~400 kcal. This washout period will be based on participants and workplace availability.
Sponsors
Victoria University
Study design
Allocation
Non-randomised trial
Intervention model
Single group
Primary purpose
Treatment
Eligibility
Sex/Gender
Male
Age
20 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
Participants will be considered relatively healthy males with the FTO rs9939609 risk allele (A) or two FTO rs9939609 wild type alleles (TT). Participants will be included if they have adequate glucose handling (no diagnosed diabetes, fasting blood glucose must be less than 7.0 mmol/L), not performing any regular fitness training (i.e. greater than 30 minutes, 3 times per week) for the past 6 months, not taking contraindicated prescription medication (i.e. thyroid, hyperlipidmeic, hypoglycemic, or antihypertensive), do have physician approval (if BMI over 25kg.m-2), and not current involvement in other research studies.
Exclusion criteria
Participants will be excluded from participating within the project for the following reasons: diagnosed diabetes (fasting blood glucose greater than 7.0 mmol/L or HbA1ctest greater than 6.5%), performing any regular fitness training (i.e. greater than 30 minutes, 3 times per week) for the past 6 months, taking contraindicated prescription medication (i.e. thyroid, hyperlipidmeic, hypoglycemic, or antihypertensive), no physician approval (if BMI over 25kg.m-2), and current involvement in other research studies.
Outcome results
None listed