None listed
Conditions
Brief summary
The aim of this project is to identify key differences in cellular pathways involved in energy metabolism at rest and following an acute bout of exercise between healthy overweight and obese and lean individuals. Forty age-matched and fitness-matched (20 lean, 20 overweight and/or obese) healthy males and females between the ages of 20 and 50 years will be recruited. Participants will perform a buccal swab and donate a venous blood sample to obtain a genetic and metabolic signature profile at rest. Participants will perform a low (40% VO2peak-LO) and high-intensity (80% VO2peak-HI) exercise bout for approximately 70 and 38 minutes, respectively, separated by 2 weeks. A DEXA scan will be performed prior to the first exercise test. Venous blood will be sampled prior to, immediately after, 15 minutes, and 3 hours following completion of the exercise. Muscle biopsies will be sampled prior to, 15 minutes and 3 hours following completion of the exercise. Urine samples will be collected prior to, 90 minutes and 3 hours following the completion of the exercise. This proposed project will allow parallel measurement of metabolism in response to exercise intensity and to be mapped against variations in genetic disposition. This will help identify and contextualize biomarkers of metabolic flexibility or inflexibility in healthy overweight and obese individuals. These biomarkers can then be used as targets for exercise, nutrition and pharmacological interventions.
Interventions
Participants will be asked to perform exercise tests during two testing sessions. One exercise test will involve a single bout of cycle ergometer exercise at 80% (HI) VO2max for 38 minutes which expends ~400 kcal). This is a crossover study with the order of the exercise tests being randomised. The second test will occur a minimum of 1 week following the commencment of first exercise test (maximum of up to 4 weeks). This washout period will be based on participant availability, work place availa
Participants will be asked to perform exercise tests during two testing sessions. One exercise test will involve a single bout of cycle ergometer exercise at 80% (HI) VO2max for 38 minutes which expends ~400 kcal). This is a crossover study with the order of the exercise tests being randomised. The second test will occur a minimum of 1 week following the commencment of first exercise test (maximum of up to 4 weeks). This washout period will be based on participant availability, work place availability, and human physiology (ie: females will be asked to perform exercise trials only within the proliferative phase of their menstral cycle).
Sponsors
Victoria University
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Prevention
Masking
Open (masking not used)
Eligibility
Sex/Gender
All
Age
20 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
Participants will be considered relatively healthy lean, overweight and obese individuals. Participants will be included if they have adequate glucose handling (no diagnosed diabetes, fasting blood glucose must be less than 7.0 mmol/L), not performing any regular fitness training (i.e. greater than 30 minutes, 3 times per week) for the past 6 months, not taking contraindicated prescription medication (i.e. thyroid, hyperlipidmeic, hypoglycemic, or antihypertensive), non-pregnant, not peri- or post-menopausal, must have physician approval (overweight and obese participants only), and not current involvement in other research studies.
Exclusion criteria
Participants will be excluded from participating within the project for the following reasons: diagnosed diabetes (fasting blood glucose greater than 7.0 mmol/L), performing any regular fitness training (i.e. greater than 30 minutes, 3 times per week) for the past 6 months, taking contraindicated prescription medication (i.e. thyroid, hyperlipidmeic, hypoglycemic, or antihypertensive), pregnant, peri- and post-menopausal women, no physician approval (overweight and obese participants only), and current involvement in other research studies.
Outcome results
None listed