The complete placental transcriptome in health and disease in pregnant Caucasian women

An observational study examining gene expression in term placentas collected from women whose pregnancies were complicated by preeclampsia or intrauterine growth restriction compared to those from healthy pregnancies.

Status

Not yet recruiting

Phases

Unknown

Study type

Observational

Source

ANZCTR

Registry ID

ACTRN12612001179820

Enrollment

600

Registered

2012-11-06

Start date

2012-11-29

Completion date

Unknown

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

We will collect placenta samples from both normal pregnancies and those complicated by preeclampsia (PE) or intrauterine growth restriction (IUGR). RNA will be extracted from these samples and will be used for whole transcriptome gene expression profiling including both coding and non-coding RNA. This will provide insight into differences between normal and PE and IUGR pregnancies in which the placenta is key to maternal and neonatal outcomes.

Interventions

We will extract RNA from placenta samples to quantify gene expression using deep sequencing by RNA-seq and microarray platforms. This is a single time-point "snapshot" observation to generate the complete placental transcriptome in health and disease. A saliva sample from both mothers and fathers will also be collected in the antenatal clinic for genome-wide SNP genotyping to determine parent of origin allele expression in placentas

Eligibility

Sex/Gender

All

Age

18 Years to 45 Years

Healthy volunteers

Yes

Inclusion criteria

Caucasian women pregnant with singleton, non-smokers, BMI 20-35. Both pregnant mothers and fathers of the pregnancy (where possible) will be asked to supply a saliva sample for genome-wide SNP genotyping.

Exclusion criteria

non caucasian ethnicity, smokers, BMI over 35 or under 20, non english speaking

Outcome results