None listed
Conditions
Brief summary
Hemodialyzer biocompatibility in terms of biofilm (secondary membrane) quantity and quality (protein composition) may depend on dialysate formula. Specifically, citrate instead of acetate might reduce the amount of protein adsorbed to dialysis membrane, stable fibrin formation as well as complement binding, and explain higher filter permeability (clearance) previously observed with citrate-based dialysate. Acetic elution of dialyzer will be performed at the end of dialysis session in a cross-over fashion (first with acetate, then with citrate), eluate protein content ascertained and factors of coagulation and complement evaluated
Interventions
Patients will be switched to citrate-based dialysate (intervention) in a cross-over fashion. The dialysate will be applied six times (thrice weekly for two weeks), patients are exposed to the dialysate for 4 hours each time. Neither base (citrate or acetate) is administered directly to the patient (dialysate is separated from the bloodstream by dialysis membrane), which makes it impossible to define the dose. That's also why it should not be considered drug intervention. Citrate concentration in
Patients will be switched to citrate-based dialysate (intervention) in a cross-over fashion. The dialysate will be applied six times (thrice weekly for two weeks), patients are exposed to the dialysate for 4 hours each time. Neither base (citrate or acetate) is administered directly to the patient (dialysate is separated from the bloodstream by dialysis membrane), which makes it impossible to define the dose. That's also why it should not be considered drug intervention. Citrate concentration in dialysate is 2.4 mmol/l.
Sponsors
Charles University in Prague
Study design
Allocation
Non-randomised trial
Intervention model
Crossover
Primary purpose
Treatment
Masking
Open (masking not used)
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
regular dialysis > 3 months native arteriovenous fistula & blood flow > 250ml/min
Exclusion criteria
history of thrombosis (other than av fistula) or anticoagulation (other than for hemodialysis) bleeding disorders
Outcome results
None listed