Does sexual inequality start in utero with sex differences in the placental transcriptome

Transcriptome-wide gene expression analysis of placentas collected at birth from male and female fetuses to evaluate the sex specific differences in human placental development.

Status

Not yet recruiting

Phases

Unknown

Study type

Observational

Source

ANZCTR

Registry ID

ACTRN12612000999831

Enrollment

300

Registered

2012-09-18

Start date

2012-10-01

Completion date

Unknown

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

We will collect placenta samples from male and female fetuses delivered by cesarean section. RNA will be extracted from these samples and will be used for whole transcriptome gene expression profiling. This will provide insight into the sex-specific differences in human placental development.

Interventions

We will be extracting RNA from placenta samples to observe gene expression using RNA-seq and microarray platforms. This is a single time-point "snapshot" observation to generate a gene expression profile.

Eligibility

Sex/Gender

All

Age

18 Years to 45 Years

Healthy volunteers

Yes

Inclusion criteria

Caucasian women pregnant with a singleton or twins who will deliver by elective cesarean. Non-smokers BMI 20-40 Fathers of the pregnancy (where possible) will asked to supply a saliva sample for genome-wide SNP genotyping.

Exclusion criteria

Severe complication of pregnancy

Outcome results