None listed
Conditions
Brief summary
There are no sensitive markers that can predict or quantify total oxidant status and total antioxidant capacity in DR patients. The lack of reliable biomarkers prevents early, cost-effective measures to target those who might benefit from intensive diabetic therapy. We aimed to observe changes of total oxidant status (TOS) and total antioxidant capacity (TAC) of aqueous humor between stages of diabetic retinopathy patients. If these biomarkers works, it will be very usefull method to detect diabetic retinopathy progression risk.
Interventions
1-Collecting aqueous humor during cataract surgery of diabetic retinopathy patients.
Aqueous humor collected just beginning of cataract surgery. corneal side insicion have been done with MVR knife and 0,1-,0,2 cc aqueous humor aspirated via 26 gauge insulin syringe then cataract surgery continued with the enjection of viscoelastic substance into anterior chamber.
2-Measuring Total antioxidant capacity and total oxidant status in collected aqueous humor.
Aqeous humor immediately stored at -80 c
1-Collecting aqueous humor during cataract surgery of diabetic retinopathy patients. Aqueous humor collected just beginning of cataract surgery. corneal side insicion have been done with MVR knife and 0,1-,0,2 cc aqueous humor aspirated via 26 gauge insulin syringe then cataract surgery continued with the enjection of viscoelastic substance into anterior chamber. 2-Measuring Total antioxidant capacity and total oxidant status in collected aqueous humor. Aqeous humor immediately stored at -80 celcius and all samples measured at the same time via Erel method. (at January 2011, one month before end of study) Measurements of total antioxidant capacity and total oxidant status performed with Erel method. It is a new calorimetric method. This method spectrophotometrically measures changes of oxidant and antioxidant ions present in the sample.
Sponsors
Ulucanlar eye research and training hospital
Study design
Allocation
Non-randomised trial
Primary purpose
Prevention
Eligibility
Sex/Gender
All
Age
35 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
Inclusion criteria included diabetic patients who had diagnosis of senile cataracts. For non-diabetic patients inclusion criteria was presence of cataract.
Exclusion criteria
Exclusion criteria included patients who had dense cataract which hinder retinopathy staging, previous ocular surgery, type 1 diabetes, history of glaucoma, retinal vein occlusion, uveitis, any other posterior segment pathologies.
Outcome results
None listed